Objective This study evaluated the lipid-lowering effect of adding citrus polymethoxyflavonoid powder to biscuits through measurement of lipid metabolism indicators in a hyperlipidemia mouse model to develop a new type functional food. Methods Sixty male KM mice were randomly divided into six groups and fed for 6 weeks to establish a high fat mouse model. Then, they were fed with the corresponding biscuits. After 6 weeks of feeding, the weight, Lee’ s index, serum lipid levels, and indexes of the liver, spleen, heart, and kidney were assessed. Results After 6 weeks, polymethoxyflavonoid biscuits inhibited the weight increase of hyperlipidemia mice and reduced hyperlipidemia. Polymethoxyflavonoid biscuits significantly improved abnormal lipid metabolism of common biochemical indicators in the serum of hyperlipidemia model mice. In terms of the effect of lipid-lowering biscuits on blood lipids in mice, the lipid- lowering biscuits had a significant effect on triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), but had no obvious effect on high-density lipoprotein cholesterol (HDL-C). Conclusions Citrus polymethoxyflavonoid powder may be a therapeutic agent for dyslipidemia, fatty liver, and other related diseases.