Pluripotent stem cells (PSCs) are a class of stem cell lines that can proliferate indefinitely and have the potential to differentiate into various tissue cells. The establishment of induced pluripotent stem cells (iPS cells) has further narrowed the distance between stem cells and clinical disease treatment, and has great potential value for applications in the fields of agricultural economic development and animal medicine. However, currently the thorough mechanism research and the application of stem cells are mainly focused on mouse and human stem cells, while the research on stem cells of large animals with a close relationship with agriculture, animal husbandry, and veterinary science is still at the preliminary establishment and application trial stage. By summarizing the relevant literature, this article briefly describes the establishment and research status of embryonic pluripotent stem cells and induced pluripotent stem cells in large animals such as pigs, cattle, and horses.