Urine metabolomics in the rat model of damp-heat diarrhea
Received:May 16, 2019  
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DOI:10. 3969 / j.issn.1005-4847. 2019. 06. 003
KeyWord:damp-heat diarrhea; UPLC-Q/ TOF-MS/ MS; urine; metabolomics; biomarkers; rat
彭晓婷 甘肃农业大学动物医学院, 兰州
马琪 西南大学动物科学学院,重庆
张晓松 甘肃农业大学动物医学院, 兰州
华永丽 甘肃农业大学动物医学院, 兰州
纪鹏 甘肃农业大学动物医学院, 兰州
姚万玲 甘肃农业大学动物医学院, 兰州
魏彦明 甘肃农业大学动物医学院, 兰州
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      Objective The ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass/ mass spectrometry (UPLC-Q/ TOF-MS/ MS) urine metabolomics method was used to screen for potential metabolic markers of rat model of damp-heat diarrhea and to explore the pathogenesis. Methods The rat model of damp-heat diarrhea was established using a high-sugar and high-fat diet (HF), a high-temperature and high-humidity environment (HH), and intraperitoneal injection of Escherichia coli (EC). Urine samples were collected from rats at different stages of modeling (HF, HH, and EC) and analyzed using UPLC-Q/ TOF-MS/ MS. The data were analyzed by multivariate statistical analysis. Results Seven potential endogenous biomarkers were screened, namely, L-glutamic acid-L-cysteine, L-formyl kynurenine, 5-hydroxy-N-formylkynurenine, riboflavin-5-phosphate, linoleic acid, L-tryptophan and melatonin. Conclusions The metabolic pathways involved in damp heat diarrhea include metabolic pathways such as tryptophan metabolism, riboflavin metabolism, and linoleic acid metabolism. The results suggest that energy-, amino acid- and lipidmetabolism disorders occur in rats with damp-heat diarrhea which provide a scientific basis for elucidating the mechanism of pathogenesis of Chinese medicine and traditional Chinese veterinary medicine damp-heat diarrhea.
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