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| Anti-atherosclerotic and cardioprotective effects of perilla seed extract on ApoE -/ - mice |
| Received:June 19, 2019 |
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| DOI：10. 3969 / j.issn.1005-4847. 2019. 06. 001 |
| KeyWord:perilla seed extract; ApoE -/ - mice; atherosclerosis; cardioprotection |
| Author | Institution |
| 郑双 |
1. 广东药科大学,生命科学与生物制药学院,血管生物学研究所,广州 ;2. 广东省实验动物监测所,广东省实验动物重点实验室,广州 |
| 谭伟江 |
广东省实验动物监测所,广东省实验动物重点实验室,广州 |
| 李想 |
广东省实验动物监测所,广东省实验动物重点实验室,广州 |
| 马俊峰 |
广东省实验动物监测所,广东省实验动物重点实验室,广州 |
| 任学聪 |
广东省实验动物监测所,广东省实验动物重点实验室,广州 |
| 郑凌云 |
广东药科大学,生命科学与生物制药学院,血管生物学研究所,广州 |
| 王丽京 |
广东药科大学,生命科学与生物制药学院,血管生物学研究所,广州 |
| 杨丰华 |
广东省实验动物监测所,广东省实验动物重点实验室,广州 |
| 黄韧 |
1. 广东药科大学,生命科学与生物制药学院,血管生物学研究所,广州 ;2. 广东省实验动物监测所,广东省实验动物重点实验室,广州 |
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| Abstract: |
| Objective To study the role and molecular mechanism of perilla seed oil extract (PSO) in reducing arterial plaque formation and providing cardioprotection in ApoE -/ - mice. Methods Three-month-old ApoE -/ - mice were randomly divided into ApoE -/ -(no drug treatment), low-dose PSO, high-dose PSO and atorvastatin groups. B6 mice of the same age were used as controls (WT group); they were fed a regular diet. After 8 weeks of treatment, biochemical tests, H&E staining, echocardiography, qPCR, ATP detection and Pro-Q Diamond staining were performed. Results Compared with the WT group, ApoE -/ - mice showed abnormal cardiac structure and function. After PSO treatment, the plaque area was reduced, and cardiac structure and function improved. mRNA expression of ANP, BNP and β-MHC was downregulated, mRNA expression of PPARα and PGC-1α was up-regulated, and the intracellular content of ATP and the phosphorylation level of TnI were increased. Conclusions PSO inhibits arterial plaque formation and maintains normal cardiac structure and function in ApoE -/ - mice. It may regulate the PPARα/ PCG-1α/ NRF-1 axis to increase ATP and myofilament phosphorylation, improve energy metabolism, and exert cardioprotective action. |
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