Protective effect of berberine on the cerebral ischemia-reperfusion injury in rats based on Bcl-2/ Beclin-1 complex
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(1. Department of Pharmacology, Chengde Medical College, Chengde 067000, China.2. Department of Immunology,Chengde Medical College, Chengde 067000)

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Q95-33

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    Abstract:

    Objective To study the neuroprotective effect of berberine on cerebral ischemia-reperfusion injury in rats based on the B cell lymphoma/ lewkmia-2 (Bcl-2) / Beclin-1 complex. Methods A total of 108 SPF grade SD ratswere randomly divided into the follow groups: sham group, model group (I/ R), berberine low, medium and high dosegroup ( BBR-L, BBR-M, BBR-H), and nimodipine pretreatment group ( NMDP group). The rats were scored forneurological function after modeling and the area of cerebral infarction was observed by TTC staining. HE staining was used to observe the his topathological changes of rat brain, and the expressions of Bcl-2, caspase-3 and Bax in brain tissues were detected by RT-PCR, while western blot was used to detect the expressions of Bcl-2, caspase-3, Bax and Beclin-1 incerebral cortex. Results The neurological function scores in the I/ R group at 6 h, 24 h and 48 h was significantly higher than that in the sham group, with the highest score at 24 h. Compared with the model group, the scores of neurologicalimpairment in the BBR group and NMDP group were significantly reduced at 24 h and 48 h ( P <0. 05). The percentage ofinfarct area and the percentage of cell necrosis in the I/ R group were increased significantly compared with the sham group( P <0. 05), while BBR group and NMDP group decreased significantly compared with the I/ R group ( P <0. 05). Bcl-2,caspase-3, Bax mRNA and protein levels in the I/ R group were significantly increased compared with the sham group. The levels of Bcl-2 mRNA and protein in the BBR group and NMDP group were increased significantly compared with the I/ Rgroup, while the levels of caspase-3, Bax mRNA and protein decreased significantly. The Bcl-2/ Bax ratio in the I/ R groupdecreased significantly compared with the sham group. The Bcl-2/ Bax ratio of each treatment group was increase dsignificantly compared with the I/ R group and was increased in a dose-dependent manner. Beclin-1 protein level and Bcl-2/Beclin-1 ratio were increased in the I/ R group compared with the sham group ( P <0. 05). The levels of Beclin-1 protein inthe BBR group and NMDP group were increased significantly compared with the I/ R group ( P <0. 05), the ratio of Bcl-2/Beclin-1 was decreased, and there was no significant difference in Bcl-2/ Beclin-1 between the BBR-M group, BBR-Hgroup and NMDP group. Conclusions Berberine has a neuroprotective effect on cerebral ischemia-reperfusion injury in rats. Berberine regulates the levels of autophagy protein and apoptotic protein, so that autophagy can reach an appropriate level and play a neuroprotective role.

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History
  • Received:April 29,2019
  • Revised:
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  • Online: November 04,2019
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