Spinal cord injury is an extremely complex and debilitating condition. Once spinal cord injury occurs, itis difficult to treat, and thus poses a great economic and social burden to both caregivers and healthcare resources. In recentyears, the establishment of rat spinal cord injury cell models has contributed to our understanding of the etiology andpathogenesis of spinal cord injury, in particular, the establishment of an astrocyte model has a profound significance for thetreatment of spinal cord injury. Studies have found that astrocytes, as target cells that can effectively promote tissueprotection and functional repair after spinal cord injury, directly or indirectly regulate spinal cord injury through blood-brainbarrier barrier. This paper reviews the recent studies on the preparation of astrocyte culture models to provide guidance forthe establishment of an Objective , quantitative, and simulative astrocyte model, and presents new ideas for the treatment of spinal cord injury.