Knockout of Tyr gene by CRISPR-Cas9 to produce albino C57BL / 6N mice
Received:February 14, 2019  
View Full Text  View/Add Comment  Download reader
DOI:10. 3969 / j.issn.1005-4847. 2019. 04. 011
KeyWord:Albino; Tyr; gene knockout; CRISPR-Cas9; C57BL/6N; mouse
常秋荣 上海交通大学医学院实验动物科学部,上海 
刘丽丽 上海交通大学医学院实验动物科学部,上海 
王会阳 上海交通大学医学院实验动物科学部,上海 
付丽 上海交通大学医学院实验动物科学部,上海 
邢凤英 上海交通大学医学院实验动物科学部,上海 
李垚 上海交通大学医学院实验动物科学部,上海 
陈学进 上海交通大学医学院实验动物科学部,上海 
李善刚 1. 上海交通大学医学院实验动物科学部,上海 ; 2. 昆明理工大学灵长类转化医学研究院,昆明 
Hits: 1686
Download times: 556
      Objective  To obtain albino C57BL/6N mice and expand their application in research of skintransplantation and embryonic stem cells. Methods Cas9 mRNA and a series of single guide RNAs (sgRNAs) weresynthesized in vitro and injected into the fertilized eggs of C57BL/6N mice. The gene encoding tyrosinase (TYR), anenzyme necessary for melanin production in C57BL/6N mice, was destroyed in exon 1 and exon 2, and gene mutations weregenerated to obtain albino F0 generation. Albino C57BL/6N mice were then subjected to repeated backcrossing andinbreeding to produce C57BL/6N albino mouse inbreds. Results Two pairs of sgRNA were injected into mice, and the F0generation of albino mice was successfully obtained. Deletions in both exon 1 and exon 2 of the Tyr gene were confirmed.The albino mice transmited the mutant gene to offsprings, and homozygous white C57BL/6N mice as offspring wereconfirmed. The mutation types of albino mice were analyzed. Conclusions The mouse Tyr gene is disrupted by CRISPRCas9technology, and the C57BL/6N albino mouse inbred line is successfully established. This mouse line provides a new research tool for future chimera preparation and tissue transplantation.
function PdfOpen(url){ var win="toolbar=no,location=no,directories=no,status=yes,menubar=yes,scrollbars=yes,resizable=yes";,"",win); } function openWin(url,w,h){ var win="toolbar=no,location=no,directories=no,status=no,menubar=no,scrollbars=yes,resizable=no,width=" + w + ",height=" + h;,"",win); } &et=E83A275BEABA9A3401ADB677797160946907586895AC73A53D63F3E3EE39AA4FE4C79CDEB5092BEEEE87F989132E6CD5764743C4335F78E442ED8620D368D5E96876AF077EA3DCCC&pcid=&cid=&jid=&yid=B6351343F4791CA3&aid=&vid=&iid=E158A972A605785F&sid=8C267C8DC97FEEEF&eid=8566B4AE2A8832E3&fileno=201904011&flag=1&is_more=0"> var my_pcid=""; var my_cid=""; var my_jid=""; var my_yid="B6351343F4791CA3"; var my_aid="";