Establishment of a tree shrew lung fibroblast model of CA16 infection and the expression of its receptor SCARB2
Received:March 28, 2019  
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DOI:10. 3969 / j.issn.1005-4847. 2019. 04. 009
KeyWord:CA16; tree shrew lung fibroblasts; Infection; SCARB2
                       
AuthorInstitution
王文广 中国医学科学院/ 北京协和医学院医学生物学研究所树鼩种质资源中心云南省重大传染病疫苗研发重点实验室;中国医学科学院医学生物学研究所实验树鼩标准化与应用研究省创新团队,昆明 
匡德宣 中国医学科学院/ 北京协和医学院医学生物学研究所树鼩种质资源中心云南省重大传染病疫苗研发重点实验室;中国医学科学院医学生物学研究所实验树鼩标准化与应用研究省创新团队,昆明 
李娜 中国医学科学院/ 北京协和医学院医学生物学研究所树鼩种质资源中心云南省重大传染病疫苗研发重点实验室;中国医学科学院医学生物学研究所实验树鼩标准化与应用研究省创新团队,昆明 
陆彩霞 中国医学科学院/ 北京协和医学院医学生物学研究所树鼩种质资源中心云南省重大传染病疫苗研发重点实验室;中国医学科学院医学生物学研究所实验树鼩标准化与应用研究省创新团队,昆明 
罕园园 中国医学科学院/ 北京协和医学院医学生物学研究所树鼩种质资源中心云南省重大传染病疫苗研发重点实验室;中国医学科学院医学生物学研究所实验树鼩标准化与应用研究省创新团队,昆明 
仝品芬 中国医学科学院/ 北京协和医学院医学生物学研究所树鼩种质资源中心云南省重大传染病疫苗研发重点实验室;中国医学科学院医学生物学研究所实验树鼩标准化与应用研究省创新团队,昆明 
孙晓梅 中国医学科学院/ 北京协和医学院医学生物学研究所树鼩种质资源中心云南省重大传染病疫苗研发重点实验室;中国医学科学院医学生物学研究所实验树鼩标准化与应用研究省创新团队,昆明 
代解杰 中国医学科学院/ 北京协和医学院医学生物学研究所树鼩种质资源中心云南省重大传染病疫苗研发重点实验室;中国医学科学院医学生物学研究所实验树鼩标准化与应用研究省创新团队,昆明 
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Abstract:
      Objective  To explore the feasibility of establishing an experimental model of tree shrew lungfibroblasts (TSLF) infected with CA16. Methods TSLF were infected with enterovirus CA16, and human lung fibroblasts(KMB-17) were used as controls. The cytopathic effects ( CPE) were observed using an inverted microscope. Theexpression of viral protein and the SCARB2 receptor protein was observed by indirect immunofluorescence assay, and viralload was detected by TaqMan real-time PCR assay. Using β-actin as an internal reference, the relative expression of theSCARB2 gene was detected using SYBR Green real-time PCR, and its correlation with the infection process was analyzedalong with the gene sequence alignment. Results CA16 infected TSLF and caused obvious CPE. The virus and SCARB2protein were observed by immunofluorescence assay. Under the infection rate of 100TCID50 /105 cells, the viral load washighest 48 h after infection. The SCARB2 gene had a high expression that was associated with infection, and its genesequence also has a high homology with that of humans. Conclusion CA16 can infect TSLF and SCARB2 is related to the infection.
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