Alterations of articular cartilage and subchondral bone in different rat models of osteoarthritis
Received:January 17, 2019  
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DOI:10. 3969 / j.issn.1005-4847. 2019. 04. 005
KeyWord:osteoarthritis, OA; animal model; articular cartilage; subchondral bone; trabecular analysis
谭启钊 北京大学第三医院骨科,北京 
牛国栋 北京脊柱疾病重点实验室,北京 
赵振达 北京大学第三医院骨科,北京 
李思维 北京大学第三医院骨科,北京 
宋纯理 北京脊柱疾病重点实验室,北京 
冷慧杰 北京大学第三医院骨科,北京 
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      Objective To study the pathological changes of subchondral bone and cartilage in different osteoarthritis(OA) models. Methods Three Sprague Dawley (SD) OA models were used and twenty-four female rats were divided into fourgroups randomly: control group (Sham, n=6), anterior cruciate ligament transection operations group (ACLT, n =6), papainjoint cavity injection group (Papain, n=6) and ovariectomy group (OVX, n=6). After 8 weeks, all knee joints were extracted.Subchondral bone specimens from tibia plateau were scanned, and trabecular parameters were obtained. Articular cartilagespecimens were stained by toluidine blue, and OA was scored according to Mankin’s histological grading system. Results After8 weeks, the OA severity of cartilage are different for different OA models. Severe cartilage damage was observed in the ACLTand Papain groups. Only mild cartilage damage was occurred in the OVX group. Subchondral bone in all OA models also changedat week 8 post operation. Subchondral trabecular bone in the OVX group became looser than the Sham group. Subchondraltrabecular bone in the ACLT group and Papain group didn’t change significantly compared with the Sham group, but hadsignificant difference from the OVX group. Thickness of subchondral bone plate in all groups became thinner than the Shamgroup. Conclusions Both subchondral bone and cartilage changed with OA progress for all the three OA animal models. Thosealterations in different OA models are different, which implies that subchondral bone might play different roles for different typesof OA. This study will provide us more evidences to further investigate mechanisms of different types of OA and more insight to treat subchondral bone as an OA therapeutic target.
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