Porcine circovirus 3 infection in BALB / c mice
Received:November 24, 2018  
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DOI:10. 3969 / j.issn.1005-4847. 2019. 03. 013
KeyWord:porcine circovirus 3; BALB/ c mice; infection; real-time PCR; ELISA; histopathology
                                      
AuthorInstitution
张超林 国家兽用药品工程技术研究中心,河南洛阳
刘攀 河南农业大学牧医工程学院,郑州
沈萌 国家兽用药品工程技术研究中心,河南洛阳
王妍 河南农业大学牧医工程学院,郑州
王娟 国家兽用药品工程技术研究中心,河南洛阳
颜世君 国家兽用药品工程技术研究中心,河南洛阳
严权斌 普莱柯生物工程股份有限公司,河南洛阳
邓均华 国家兽用药品工程技术研究中心,河南洛阳
孙哲 国家兽用药品工程技术研究中心,河南洛阳
郭小参 普莱柯生物工程股份有限公司,河南洛阳
王同燕 国家兽用药品工程技术研究中心,河南洛阳
李向东 国家兽用药品工程技术研究中心,河南洛阳
田克恭 1. 国家兽用药品工程技术研究中心,河南洛阳 ; 2.河南农业大学牧医工程学院,郑州
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Abstract:
      Objective To study porcine circovirus 3 (PCV3) infection in BALB/ c mice. Methods FemaleBALB/ c mice were divided into two groups. Mice in the experimental group were infected with PCV3 derived from infectedpig tissues. The mice in the control group were injected with the same volume of PBS. The status of the mice was observeddaily after infection, and blood samples were analyzed by quantitative RT-PCR and ELISA at days 0, 3, 7, 11 and 14 postinfection. At the end of the experiment, all animals were euthanized, and samples of heart, liver, spleen, lung, kidney,brain and lymph nodes were taken for quantitative RT-PCR and pathological examination. The PCV3 CAP gene wassequenced in the PCR-positive tissues. Results Mice infected with PCV3 did not show obvious clinical symptoms orpathological changes, and the virus was detected in serum during early infection. The liver and spleen showed the highestviral copy numbers, and the nucleic acid sequence was not changed in the infected organs. Antibody titers were increasedduring the infection. Conclusions BALB/ c mice can be infected with PCV3. This study provides useful information to study the pathogenicity of PCV3 as well as prevention and control of the disease.
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