Phenotype and pathological observation of leptin receptor-knockout SD rats
Received:December 12, 2018  
View Full Text  View/Add Comment  Download reader
DOI:10. 3969 / j.issn.1005-4847. 2019. 03. 010
KeyWord: Lepr -/ - rats; obesity; metabolic syndrome; lipidosis
                    
AuthorInstitution
蒋丹丹 河北医科大学基础医学院病理教研室,石家庄
曹兰凤 河北医科大学基础医学院病理教研室,石家庄
许朝霞 河北医科大学基础医学院病理教研室,石家庄
李硕 河北医科大学基础医学院病理教研室,石家庄
董晓辉 河北医科大学新药安全评价研究中心,石家庄
姚芳 河北医科大学基础医学院病理教研室,石家庄
张连珊 1. 河北医科大学基础医学院病理教研室,石家庄 ;2. 河北医科大学新药安全评价研究中心,石家庄
Hits: 1616
Download times: 609
Abstract:
      Objective To observe the various parameters related to metabolic syndrome and prediabetes in aLeptin receptor-knockout ( Lepr -/ - ) rat model established by CRISPR/ Cas9 technique. Methods Periodically, wemeasured the weights, daily food intake, random and fasting blood glucose, and serum biochemical indexes of the Lepr -/ -rats, and performed glucose and insulin tolerance tests. Pathological changes in the main organs of Lepr -/ - rats wereexamined at 14 and 18 weeks of age. We also observed the lipid deposition in the main organs of Lepr -/ - rats. Results Lepr -/ - rats exhibited obesity, high food intake, dysfunctional glucose tolerance and insulin resistance as well asdyslipidemia. Islet and myocardial hypertrophies, fatty liver, and obesity-related glomerulopathy were observed in the Lepr -/ - rats at 14 and 18 weeks of age. Lipid deposition was found in hepatocytes, renal tubular epithelial cells, and skeletalmuscle cells. Conclusions Lepr -/ - rats are an ideal animal model of metabolic diseases and may contribute to revealing the pathogenesis and new drug development for such metabolic disorders.
Close
function PdfOpen(url){ var win="toolbar=no,location=no,directories=no,status=yes,menubar=yes,scrollbars=yes,resizable=yes"; window.open(url,"",win); } function openWin(url,w,h){ var win="toolbar=no,location=no,directories=no,status=no,menubar=no,scrollbars=yes,resizable=no,width=" + w + ",height=" + h; controlWindow=window.open(url,"",win); } &et=F35444EE904EDFFF960F7A767165B6EFF07CEF79A50BC2DBD33C35016D18AD12FB02C2EA68607278D145BB206FCCC3B2B0661579B6DBC7CBC7D6BDED52C3615BD664BA37CED5FB865582995208103B4E&pcid=&cid=&jid=&yid=B6351343F4791CA3&aid=&vid=&iid=38B194292C032A66&sid=717CC18E05F2AFA0&eid=302684F6FB4B24FF&fileno=201903010&flag=1&is_more=0"> var my_pcid=""; var my_cid=""; var my_jid=""; var my_yid="B6351343F4791CA3"; var my_aid="";