Phenotype and pathological observation of leptin receptor-knockout SD rats
Received:December 12, 2018  
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DOI:10. 3969 / j.issn.1005-4847. 2019. 03. 010
KeyWord: Lepr -/ - rats; obesity; metabolic syndrome; lipidosis
蒋丹丹 河北医科大学基础医学院病理教研室,石家庄
曹兰凤 河北医科大学基础医学院病理教研室,石家庄
许朝霞 河北医科大学基础医学院病理教研室,石家庄
李硕 河北医科大学基础医学院病理教研室,石家庄
董晓辉 河北医科大学新药安全评价研究中心,石家庄
姚芳 河北医科大学基础医学院病理教研室,石家庄
张连珊 1. 河北医科大学基础医学院病理教研室,石家庄 ;2. 河北医科大学新药安全评价研究中心,石家庄
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      Objective To observe the various parameters related to metabolic syndrome and prediabetes in aLeptin receptor-knockout ( Lepr -/ - ) rat model established by CRISPR/ Cas9 technique. Methods Periodically, wemeasured the weights, daily food intake, random and fasting blood glucose, and serum biochemical indexes of the Lepr -/ -rats, and performed glucose and insulin tolerance tests. Pathological changes in the main organs of Lepr -/ - rats wereexamined at 14 and 18 weeks of age. We also observed the lipid deposition in the main organs of Lepr -/ - rats. Results Lepr -/ - rats exhibited obesity, high food intake, dysfunctional glucose tolerance and insulin resistance as well asdyslipidemia. Islet and myocardial hypertrophies, fatty liver, and obesity-related glomerulopathy were observed in the Lepr -/ - rats at 14 and 18 weeks of age. Lipid deposition was found in hepatocytes, renal tubular epithelial cells, and skeletalmuscle cells. Conclusions Lepr -/ - rats are an ideal animal model of metabolic diseases and may contribute to revealing the pathogenesis and new drug development for such metabolic disorders.
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