Expression and significance of thrombomodulin in a rat model of deep-vein thrombosis
Received:November 21, 2018  
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DOI:10. 3969 / j.issn.1005-4847. 2019. 02. 011
KeyWord:thrombomodulin; deep vein thrombosis; thrombi; regression; rats
成曦 南通大学医学院,南通
孙宝兰 南通大学医学院,南通
苏张瑶 南通大学医学院,南通
张玉泉 南通大学附属医院妇产科,南通
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      Objective To observe the expression and explore the significance of thrombomodulin(TM) in theformation and regression of deep vein thrombosis (DVT) in rats. Methods Sprague-Dawley rats were divided randomly intotwo groups: model (n = 60) and control (n = 6) groups. A DVT model was established by stenosis (“stenosis method”)in the model group and a sham operation was performed in the control group. The rats were killed at 1, 4, 6, 12, 24 h aswell as 3, 7, 14, 21 and 28 days after modeling. The weight:length ratio of thrombi, plasma concentration of solubleTM, expression of TM mRNA in the thrombi and inferior vena cava (IVC) endothelium were measured. Results The weight:length ratio of thrombi was low in the early stage of modeling (1, 4, 6, 12 h), showing no significant differenceamong the four subgroups ( P > 0. 05). The model was stable at a higher level 24 h, 3 days and 7 days after modeling ( P >0. 05). It began to decrease 14 days after modeling, but there was no significant difference among the results at 14, 21 or28 days ( P > 0. 05). The ELISA showed that the concentration of soluble TM was significantly higher than that in thecontrol group ( P < 0. 01), and that the level of soluble TM in plasma increased gradually. The real-time fluorescencequantitative polymerase chain reaction showed that the mRNA expression of TM in thrombi increased gradually over time.Expression of TM mRNA in IVC endothelium was significantly higher at 1, 4, 6 and 12 h than that in the control group ( P < 0. 01) and significantly lower than that in the control group at 24 h, 3 days and 7 days ( P < 0. 01). There was nosignificant difference in expression of TM mRNA at 14, 21, or 28 days compared with that in the control group ( P >0. 05). A negative correlation between the weight:length ratio of thrombi and expression of TM mRNA in the IVCendothelium was determined (r = - 0. 92, P < 0. 01). However, expression of TM mRNA in thrombi was positivelycorrelated with the levels of soluble TM in plasma (r= 0. 96, P < 0. 01). Conclusions Our findings suggest that TM isinvolved in the entire process of the formation and regression of thromb, and the changes in endothelial TM expression mayreflect the occurrence and development of deep vein thrombosis,and is of a good prognostic significance.
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