Bifidobacteria alleviate chronic alcoholic liver injury in rats by regulating SIRT1/ ChREBP expression
Author:
Affiliation:

(1. Department of Pharmacy, the First Affiliated Hospital of Xiamen University, Xiamen 361003, China.2. Department of Gastroenterology, Fujian Provincial Hospital, Fuzhou 350001)

Clc Number:

Q95-33

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    Objective To investigate the protective effect of Bifidobacterium (BIFI) on liver function in rats with chronic alcoholic liver injury (CALI) and to explore the mechanism involved. Methods SD rats were randomly divided into a CALI group, metadoxine (90 mg/ kg) group, BIFI low- (500 mg/ kg), medium- (1000 mg/ kg), and high-dose(2000 mg/ kg) groups, and SIRT1 inhibitor Tenovin-6 (25 mg/ kg) group. The CALI group and a blank control group were given equal volumes of normal saline. After 8 weeks, liver function of each group was analyzed. Levels of TG and TC in liver tissue and serum were determined. The pathological changes of liver tissue were observed by hematoxylin-eosin staining. The expression of SIRT1 and chREBP in liver tissue was analyzed by western blotting. Results Compared with the control group, the liver function of the rats in the CALI group decreased significantly, and the levels of ALT and AST in the blood increased significantly ( P < 0. 05). In addition, liver tissue underwent fatty pathological damage. The levels of TG and TC in liver tissue and serum were significantly increased ( P < 0. 05), the expression of SIRT1 protein was significantly decreased ( P < 0. 05), and the expression of chREBP protein was significantly increased ( P < 0. 05).Compared with the CALI group, the liver function of the rats in the low-, middle-, and high-dose BIFI groups was significantly enhanced. The levels of ALT and AST in the blood were also significantly decreased ( P < 0. 05), the pathological damage of liver tissue was significantly reduced, and the levels of TG and TC in liver tissue and serum were significantly decreased ( P < 0. 05), the expression of SIRT1 protein was significantly increased ( P < 0. 05), and the expression of chREBP protein was significantly decreased ( P < 0. 05). All of the above effects could be reversed by the SIRT1-specific inhibitor Tenovin-6. Conclusions BIFI may inhibit the accumulation of lipids by regulating SIRT1/ChREBP expression and protect rats from chronic alcoholic liver injury.

    Reference
    Related
    Cited by
Get Citation
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:July 17,2018
  • Revised:
  • Adopted:
  • Online: January 03,2019
  • Published: