Establishment of a model of fungal keratitis in tree shrew and the effects of mannose receptor and TNF-α on inflammation
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(Center of Tree Shrew Germplasm Resources, Institute of Medical Biology, the Chinese Academy of Medical Science and Peking Union Medical College. Yunnan Innovation Team of Standardization and Application Research of Tree Shrew. the Key Laboratory of Yunnan Province for Ophthalmic Research and Disease Control. Kunming 650118, China)

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Q95-33

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    Abstract:

    Objective To determine the changes in the expression of mannose receptor and some inflammatory cytokines in the cornea of tree shrews infected by Fusarium solanum, and explore the mechanism of fungal infection keratitis. Methods A tree shrew cornea was infected by Fusarium solanum using a contact lens-assisted method, The animal model was evaluated by anterior segment photography, analysis of pathological sections, and in vivo confocal microscopy. qPCR was used to determine the expression of MR (mannose receptor), dectin-1, IL-1β, IL-6, and TNF-α in the tree shrew keratitis. The primary corneal cells of tree shrews were cultured and an infection experiment was carried out in vitro. Results The success rate of establishing the tree shrew fungal keratitis model was 84%. The positive expression of MR and dectin-1 at the early stage of infection caused peaks in the expression of IL-1β, IL-6, and TNF-α;the expression of these factors was positively correlated with the severity of fungal keratitis in the tree shrews. There was no significant change in corneal epithelial cell infection before and after infection ( P > 0. 05). Conclusions The model of fungal keratitis in tree shrew is successfully established, and its symptoms are similar to those of human fungal keratitis.Type C lectin receptors may be involved in regulating the immune response to fungal infection. The immune system may play an important role in the development and progression of keratitis.

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History
  • Received:September 26,2018
  • Revised:
  • Adopted:
  • Online: January 03,2019
  • Published: