Effects of N-acetylcysteine on oxidative stress and FoxO1 activity of the liver in type 2 diabetic rats
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(Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan 430060, China)

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Q95-33

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    Abstract:

    Objective To investigate the effects of antioxidant N-acetylcysteine (NAC) on oxidative stress and FoxO1 activity of the liver in type 2 diabetic rats. Methods Twenty-four male SD rats were randomly divided into control(C), diabetes without treatment (D), and NAC-treated diabetic groups (D + NAC) (n =8). A model of type 2 diabetes was established by feeding on a high-fat diet and the intraperitoneal injection of low-dose streptozotocin (STZ). NAC was given at a dose of 1. 5 (kg·d), and groups C and D were given an equal volume of normal saline by gavage for 8 weeks.The levels of plasma triglyceride (TG), free fatty acid (FFA), glutamic oxaline aminotransferase (AST), and alanine transaminase (ALT) were measured by an automatic biochemical analyzer, and the levels of dismutase (SOD), catalase(CAT), glutathione peroxidase (GSH-Px), and adenosine 5′-triphosphate (ATP) in liver tissues and malondialdehyde(MDA) in plasma and liver tissues were detected by commercial kits. Caspase-3 expression and FoxO1 activity in liver were analyzed by Western blot. Results The plasma levels of TG, FFA, AST, ALT, and MDA, and hepatic MDA production, caspase-3 expression, and FoxO1 activity in D group were significantly increased as compared with those in C group, while the hepatic activities of SOD, CAT, and GSH-Px, as well as ATP levels were significantly decreased. All of these changes in diabetic rats were significantly attenuated by NAC treatment for 8 weeks. Conclusion Antioxidant NAC attenuates diabetes-related liver dysfunction, partially as a result of reducing oxidative stress, mitochondrial dysfunction,and FoxO1 activation.

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History
  • Received:May 01,2018
  • Revised:
  • Adopted:
  • Online: January 03,2019
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