Objective To compare the characteristics of massive tibial bone defect (MTBD) models and induced membrane established by the internal fixation plate (IFP), intramedullary pin (IMP), and circular external fixator (CEF)based on Masquelet technology and selecting the optimal model method. Methods A total of 60 10-week-old SD male rats of SPF grade were randomly divided into three groups,namely, the IFP group, IMP group, and CEF group. A 4-mm bone defect model was generated at the middle of the right tibia, which was fixed with a custom-made six-hole stainless steel plate, a 1-mm-diameter Kirschner wire, and a custom-made circular external fixator, respectively. The time of modeling,the amount of bleeding, and the duration of limb swelling were recorded. X-ray examination was performed to observe the stability of bone cement and fixation devices. HE staining was conducted to observe the histomorphological characteristics.Results In terms of modeling time, bleeding volume, and model success rate, the IMP and CEF groups were significantly better than the IFP group, and significant differences were observed( P < 0. 05). The duration of limb swelling was the shortest in the CEF group, but no significant difference was found ( P > 0. 05). In addition, one case of screw loosening and three cases of bone cement loosening occurred in the IFP group, and 1 case of cement loosening occurred in the CEF group, whereas bone cement was well fixed in the IMP group. With regard to infection, there were three cases of plate exposure in the IFP group, two cases of purulent mass in the IMP group, and no infection in the CEF group. The thickness of induced membrane in the three groups was 460 -520 μm, showing no significant difference ( P >0. 05). Conclusions Taking the findings together, the three fixation models can successfully construct the MTBD model, but CEF is optimal for constructing the MTBD model by simulating the Masquelet technology.