Correlation between gut microbiota and anti-dsDNA antibody in the mouse model of systemic lupus erythematosus
Received:December 29, 2017  
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DOI:10.3969/j. issn. 1005 -4847. 2018. 02. 009
KeyWord:gut microbiota; systemic lupus erythematosus; Anti-dsDNA antibody; mice
邹桂香 南昌大学第二附属医院风湿免疫科,南昌
段新旺 南昌大学第二附属医院风湿免疫科,南昌
牛海涛 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,北京
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       Objective To explore the relationship between gut microbiota and anti-dsDNA antibody in systemic lupus erythematosus (lupus) TC mice. Methods ELISA was performed to detect serum anti-dsDNA antibody in the lupus TC mice. Then, the feces of both dsDNA-positive and -negative mice were collected, and 16S rRNA of the stool sample was sequenced by Illumina HiSeq 2500 high-throughput sequencing, to analyze the relationship between gut microbiota and anti-dsDNA antibody level in the two groups. Results The result of ELISA and mouse fecal high-throughput sequencing showed that the species diversity of gut microbiota in the dsDNA-positive TC mice was significantly lower than that in the dsDNA-negative TC mice. The gut microbiota of TC mice in the two groups showed significant differences at different classification levels: Chloroflexi at phylum level, Betaproteobacteria, Deltaproteobacteria and Ktedonobacteria at class level, Burkholderiales, Desulfovibrionales and Ktedonobacterales at order level, Alcaligenaceae and Desulfovibrionaceae at family level, and Parasutterella and Desulfovibrio at genus level ( P <0.05 for all). Conclusions The flora composition of gut microbiota in lupus TC mice is highly correlated with anti-dsDNA antibody. The results of our study provide a basis for further elucidation of the relationship between gut microbiota and the pathogenetic mechanism of systemic lupus erythematosus.
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