Comparative analysis of intestinal flora of Microtus fortis living under laboratory feeding and wild survival conditions
Received:September 05, 2017  
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DOI:10.3969/j. issn. 1005 -4847. 2018. 02. 008
KeyWord:Microtus fortis; intestinal flora; 16S rDNA; bacterial diversity
冯洁 上海实验动物研究中心,上海
沈志敏 上海实验动物研究中心,上海
王胜昌 上海实验动物研究中心,上海
林金杏 上海实验动物研究中心,上海
柏熊 上海西普尔-必凯实验动物有限公司,上海
谢建云 上海实验动物研究中心,上海
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      Objective To compare the differences of bacterial distribution of intestinal flora in Microtus fortis living under laboratory feeding and wild survival conditions. Methods The 16S rDNA?V4?V5 region of bacteria in the ileocecal contents from Microtus fortis raised in lab and captured in wild were measured by high?throughput sequencing. The number of operational taxonomic units (OTUs) were sorted and calculated, and the species abundance and distribution and difference were analyzed. Results The rarefaction curves indicated that adequate sampling was achieved. At the phylum level, the distribution of intestinal flora between two groups was similar. The experimental group had a unique phylum, Lentisphaerae. The wild type group had 3 unique phylums, Fusobacteria, Thaumarchaeota and an unclassified phylum. At the genus level, the kind of intestinal flora in the wild type group was more abundant than the experimental group. Ruminococcus is the largest differential genus. Conclusions The microbial community structure and differences of Microtus fortis living under different conditions are obtained. It may further enrich the basic biology data of Microtus fortis.
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