Effect of leptin on expression of lipoic acid synthase in the liver and kidney of Lepr db/db mice
Received:October 06, 2017  
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DOI:10.3969/j. issn. 1005 -4847. 2018. 02. 002
KeyWord:Lepr db/db mice; lipoic acid; liver; kidney; leptin
彭强 新乡医学院公共卫生学院,河南新乡
赵英政 新乡医学院公共卫生学院,河南新乡
闫婷婷 新乡医学院公共卫生学院,河南新乡
翟晓楠 新乡医学院公共卫生学院,河南新乡
张旭旭 新乡医学院公共卫生学院,河南新乡
易宪文 新乡医学院公共卫生学院,河南新乡
张合喜 新乡医学院公共卫生学院,河南新乡
徐光翠 新乡医学院公共卫生学院,河南新乡
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      Objective To study the expression of lipoic acid synthase (LIAS) in the liver and kidney of Lepr db/db mice with deficient leptin receptor. Methods Eight 10?week old male Lepr db/+ mice and Lepr db/db mice were included in this study. The body weight of rats in the two groups was measured. Fasting blood glucose (FPG) was measured with blood glucose test strips for all mice after fasting for 8 hours. Blood samples were obtained from the abdominal aorta and the animals were sacrificed. The liver and kidney were weighed. The right lobe of liver and the left kidney samples were fixed in 4% paraformaldehyde for pathological examination. Serum samples were separated and the sereum contents of CHO, TG, HDL and LDL were detected. The mitochondria of liver and kidney tissues were extracted with a mitochondrial isolation kit, and the protein was extracted. The expression of LIAS protein was detected by western blot. Results Histopathological observation showed that the liver and kidney tissues of Lepr db/+ mice have intact and clear structure. But the liver tissue of Lepr db/db mice showed fatty degeneration, the kidney tissue showed glomerular hypertrophy, basement membrane thickening, mesangial area widened, including mesangial cells and mesangial matrix increased. The GLU, CHO, TG, LDL and AST of Lepr db/db mice were significantly increased compared with those of Lepr db/+ mice ( P < 0.05). Compared with Lepr db/+ mice, the LIAS protein expression was significantly increased in the liver and kidney mitochondria of Lepr db/db mice ( P <0.05). Conclusions There is impaired glucose and lipid metabolism in the Lepr db/db mice which has defect leptin receptor, and the expression of LIAS protein in liver and kidney of the Lepr db/db mice is higher than that of Lepr db/+ mice.
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