Establishment of a C57BL / 6 J mouse model of metastatic melanoma in the lung
Received:October 12, 2017  
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DOI:10.3969/j. issn. 1005 -4847. 2018. 02. 001
KeyWord:C57BL/6 J mice; mouse melanoma cell line B16F10; lung metastasis; tumor model
                       
AuthorInstitution
孟星君 苏州大学附属第一医院,江苏省血液研究所,卫生部血栓与止血重点实验室,血液学协同创新中心,江苏苏州
李孝东 苏州大学附属第一医院,江苏省血液研究所,卫生部血栓与止血重点实验室,血液学协同创新中心,江苏苏州
刘俊 苏州大学附属第一医院,江苏省血液研究所,卫生部血栓与止血重点实验室,血液学协同创新中心,江苏苏州
周康熙 苏州大学附属第一医院,江苏省血液研究所,卫生部血栓与止血重点实验室,血液学协同创新中心,江苏苏州
崔庆亚 苏州大学附属第一医院,江苏省血液研究所,卫生部血栓与止血重点实验室,血液学协同创新中心,江苏苏州
胡仁萍 苏州大学附属第一医院,江苏省血液研究所,卫生部血栓与止血重点实验室,血液学协同创新中心,江苏苏州
闫荣 苏州大学附属第一医院,江苏省血液研究所,卫生部血栓与止血重点实验室,血液学协同创新中心,江苏苏州
戴克胜 苏州大学附属第一医院,江苏省血液研究所,卫生部血栓与止血重点实验室,血液学协同创新中心,江苏苏州
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Abstract:
      Objective To investigate the influencing factors involved in the establishment of a C57BL/6 J model of metastatic melanoma in the lung, including the way of tumor inoculation, the number of inoculated cells and the time of tumor formation. Methods Mouse melanoma B16F10 cells were cultured in vitro. 1) Eighteen healthy male C57BL/6 J mice were randomly divided into three groups. Mice in each group received 100 μL cell suspension (including 3 × 106 melanoma cells) via intravenous, intraperitoneal and subcutaneous injection, respectively. After two weeks, the mice were killed and dissected, and the tumor growth and metastasis were observed. 2) Eighteen male mice were randomly divided into three groups. Mice in each group were injected with 3 × 106 cells, 1 × 106 cells, and 3 × 105 cells through the tail vein, respectively. After two weeks, mice were killed and dissected, and the tumor growth and metastasis were observed. 3) Eighteen male mice were randomly divided into three groups. Mice in each group were injected with 1 ×106 cells though the tail vein. Mice were killed and dissected after one week, two weeks and three weeks, respectively. The growth and metastasis of tumor were observed. Results 1) The success rate of lung metastasis was 100% in the mice with intravenous injection, but not in mice receiving intraperitoneal injection and subcutaneous injection. 2) The size of metastatic melanoma nodules were moderate in mice inoculated by 1 ×106 cells. The number of melanoma metastatic foci was too high in the mice inoculated with 3 ×106 cells, but too low in the mice inoculated with 3 ×105 cells. 3) Significant metastatic melanoma foci were observed in the mice killed and dissected after two weeks with no death. The number of melanoma foci in the lung was too high in the mice killed after three weeks, while was too low in the mice killed at one week after tumor cell inoculation. Conclusions Intravenous injection of 1 ×106 mouse melanoma cells into C57BL/6 J mice and killed after two weeks is an optimal method for establishment of a mouse model of metastatic melanoma in the lung, and is worth of recommendation.
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