Objective To establish a rat xenograft model of human uterine leiomyoma using immunosuppressive agent and provide a useful tool for the study on uterine leiomyoma. Methods Intragastric administration with immunosuppressive agent mycophenolate mofetil (MMF) (40 mg/ kg) was given to rats for two weeks before the surgery until the end of the experiment. 20 SPF female Wistar rats were randomly divided into 4 groups after abdominal transplantation of human leiomyoma tissues: group A received femoston containing 0.4 mg/ kg estradiol and 2 mg/ kg dydrogesterone, group B received estadiol 0.4 mg/ kg, group C received dydrogesterone 2 mg/ kg, and group D served as the control group, received distilled water 1 mL/200 g. All rat received the corresponding drugs once per day for 2 days. Samples were taken at 4 weeks after the surgery to observe the pathology of the tumor tissues. Results The modeling success rates were 90% in the group A, 40 % in the group B, and 0% in the groups C and D. Conclusions Rat xenograft model of human leiomyoma can be successfully established using an immunosuppressive agent femostone with a high modeling success rate and low cost. It can be used as a new animal model for the study of transplanted leiomyoma.