Objective To investigate the efficacy and safety of the affinity adsorption material for removal of intestinal endotoxin in the blood. Metheds To establish a canine model of intestine-derived endotoxemia by ligation and perforation of the appendix. Hemoperfusion was performed to treat the endotoxemia in model dogs. In the experiment, we monitored vital signs and detect the content of endotoxin and blood physiological and biochemical indexes at different time points. Results The endotoxin content was (0.49 ± 0.22), (0.034 ± 0.00) Eu/ mL after hemoperfusion for 1 and 2 hours, compared with that before the beginning of perfusion(7.25 ±1.18)Eu/ mL, showing a significant difference ( P <0.05). After treatment for 2 hours, the average clearance rate was 99.52%. White blood cells, red blood cells, hemoglobin, platelets, total protein, albumin, globulin, alanine aminotransferase, aspartate aminotransferase, urea and creatinine index were significantly decreased after perfusion ( P < 0.05). The index values were in a normal range and vital signs were stable during the perfusion. Conclusions The affinity adsorption material developed by our team can effectively remove endotoxin in the blood of experimental dogs and has good blood compatibility.