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| Establishment of an anxiety-like gastric hypersensitivity rat model of functional dyspepsia induced by a new method of sequential stress |
| Received:March 27, 2017 |
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| DOI：10.3969/j.issn.1005-4847.2017.05.015 |
| KeyWord:Sequential stress;Anxiety;Gastric hypersensitivity;Functional dyspepsia;Rat model |
| Author | Institution |
| 景富春 |
西安交通大学第二附属医院消化内科, 西安 ;延安大学附属宝鸡市人民医院消化内科, 陕西 宝鸡 |
| 张军 |
西安交通大学第二附属医院消化内科, 西安 |
| 冯诚 |
西安交通大学第二附属医院消化内科, 西安 |
| 年媛媛 |
西安交通大学第二附属医院消化内科, 西安 |
| 杨宝德 |
西安交通大学医学部药理学系, 西安 |
| 胡浩 |
西安交通大学医学部药理学系, 西安 ;西安交通大学医学部基础医学实验教学中心, 西安 |
| 孙晓明 |
西安交通大学医学部基础医学实验教学中心, 西安 |
| 郑建云 |
西安医学院第一附属医院病理科, 西安 |
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| Abstract: |
| Objective To establish an anxiety-like gastric hypersensitivity (GHS) rat model of functional dyspepsia induced by new sequential stress. Methods Twenty-six male 1-day-old Sprague-Dawley rat pups were randomly divided into control and model groups (n=13 in each group). The model rats received sequential stress from postnatal day 2:neonatal maternal separation (NMS), acute gastric irritation (AGI) and restraint stress (RS). The control rats were reared freely with their mothers without undergoing the sequential stress. From postnatal 8th week all rats started to receive elevated plus maze (EPM), open field test (OF), abdominal withdrawal reflex (AWR) and electromyographic test (EMG).Results EPM and OF experiments depicted that the model rats showed obvious anxiety-like behavior (P<0.05 or P<0.01). AWR and EMG tests exhibited that the model rats had elevated gastric hypersensitivity to gastric distention (P<0.05 or P<0.01).Conclusions An anxiety-like GHS rat model of functional dyspepsia can be successfully established with our new method of sequential stress. |
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