Establishment and evaluation of a guinea pig model of itching
Received:March 28, 2017  
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DOI:10.3969/j.issn.1005-4847.2017.05.011
KeyWord:Histamine;4-aminopyridine;Guinea pig;Itching model
                    
AuthorInstitution
米本中 重庆市中药研究院实验动物研究所,重庆
杨武斌 重庆市中药研究院实验动物研究所,重庆
罗晓玲 重庆市中药研究院实验动物研究所,重庆
王旖 重庆市中药研究院实验动物研究所,重庆
胡娟 重庆市中药研究院实验动物研究所,重庆
徐嘉红 重庆市中药研究院实验动物研究所,重庆
樵星芳 重庆市中药研究院实验动物研究所,重庆
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Abstract:
      Objective To optimize the optimal doses of histamine and 4-aminopyridine (4-AP) in the establishment of guinea pigs models of itching, and to establish a new guinea pig model of itching. Methods The central composite design-response surface method was used to arrange the experiment. In the experiment different pruritus agents were hypodermically injection of 0.5 mL in the depilated area, and the scratching incubation period and scratching number in 30 minutes were counted after the injection. The guinea pig itching model was evaluated by observing the behavioral changes of guinea pigs and measuring the levels of histamine and interleukin-6 in the blood.Results The behavioral experiments found that the scratching frequency in the the combination group was significantly higher than the histamine group and 4-AP group (P<0.01). The itching latency of the combination group was significantly shorter than that of the histamine group and 4-AP group (P<0.01). Compared with the control group, the histamine concentrations of the combination group and histamine group were significantly increased (P<0.05 or P<0.01), and the level of the combination group was lower than that of the histamine group (P<0.05). Compared with the control group, the serum IL-6 concentrations of histamine group, 4-AP group and combination group were significantly higher (P<0.01 or P<0.05), and those in the combination group were significantly higher than the histamine and 4-AP groups. Compared with the control group, pathologic examination showed proliferation of inflammatory cells in all model groups, and the reaction of the combination group was more obvious.Conclusions The optimal conditions used in this experiment are easy to achieve and have good reproducibility in the establishment of a guinea pig model of itching.
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