Effect of single intraosseous injection of simvastatin on neoangiogenesis in myocardial infarction in rats
Received:April 19, 2017  
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KeyWord:Intraosseous injection;Simvastatin;Neovascularization;Myocardial infarction;Endothelial progenitor cells
刘灿 北京大学第三医院骨科,北京
海宝 北京大学第三医院骨科,北京
张稳 北京大学第三医院骨科,北京
祝俊雄 北京大学第三医院骨科,北京
王红 北京大学第三医院骨科,北京 ;脊柱疾病研究北京市重点实验室,北京
李子健 北京大学第三医院心内科,北京
徐迎胜 北京大学第三医院神经内科,北京
宋纯理 北京大学第三医院骨科,北京 ;脊柱疾病研究北京市重点实验室,北京
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      Objective To explore the effect of single local intraosseous injection of small dose simvastatin on the angiogenesis and cardiac function in rats after myocardial infarction. Methods Adult male Wistar rats were divided into sham operation group, myocardial infarction model group and intraosseous injection of simvastatin 0.5 mg group (all n=12 per group). The left anterior descending branch of coronary artery was ligated to establish a rat model of myocardial infarction. The left ventricular function was evaluated by small animal echocardiography at 4 weeks postoperatively. The rest of the rats were sacrificed, the myocardial infarct size was evaluated by TTC staining, and the myocardial neovascularization was detected by immunofluorescence staining.Results We successfully established the rat model of myocardial infarction. The echocardiography showed that the left ventricular systolic function was decreased significantly at 4 weeks after myocardial infarction. Intraosseous injection of simvastatin (0.5 mg) did not improve the left ventricular function after myocardial infarction in the rats. TTC staining showed that intraosseous injection of simvastatin did not reduce myocardial infarct size. Immunofluorescence staining showed that the myocardial capillary density of simvastatin group was slightly higher than that of myocardial infarction model group, but showing no significant difference between them.Conclusions Intraosseous injection of simvastatin 0.5 mg 24 hours after myocardial infarction cannot significantly promote myocardial angiogenesis, which is believed to be beneficial to the revascularization after ischemia, and thus failed to improve the cardiac function.
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