Objective To establish a mouse model of systemic C. albicans infection by oral inoculation of the pathogen and observe the proliferation and distribution of C. albicans in vivo tissues.Methods Male ICR mice(n=46)were used as the experiment group(n=40) and blank group (n=6). Cotton swabs with C. albicans were used to infect the mice (7×10⁶ CFU/mL), and the blank group with saline. The mice of the experiment group were randomly divided into two groups:model group A for clinical assessment (n=20) and model group B for tissue fungal burden detection (n=20). Clinical score, survival and autopsy were carried out among the model group A.Five mice were randomly killed from the model group B at 3 d, 5 d and 7 d after infection, respectively (blank group killed 2 mice each time). Microbial load tablet method was used to detect the tissue fungal burdens in different tissues, meanwhile samples of tongue, esophagus, stomach, liver, kidney, lung of infected mice were taken for pathological examination.Results White spot appeared on the surface of tongue since 3 d postinfection and increased with time and finally caused death.The mortality reached over 50% at 5 d. C.albicans was not only detected from the tongue (87.5%), stomach (87.5%), liver (54.5%), kidney (50.5%), lung (20%) and heart (4%), but also was microscopically seen mycelia proliferation in the tongue, stomach, liver, and kidney, yet not seen in the control group, showing that C.albicans caused disseminated systemic infection through mucosal infection in mice.Conclusions C.albicans can induce opportunistic systemic infection by breakthrough the mucosal immune barrier, so as to increase the infection to death.