Mutagenicity evaluation of the hydrolysate of Meretrix meretrix Linnaeus soft tissue
Received:February 29, 2016  
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DOI:10.3969/j.issn.1005-4847.2016.05.015
KeyWord:Meretrix Meretrix Linnaeus;Mutagenicity;Ames test;Chromosome aberration assay;Micronucleus assay
              
AuthorInstitution
高梅 山东省药学科学院, 山东省化学药物重点实验室, 济南
曹冲 山东省药学科学院, 山东省化学药物重点实验室, 济南
王功霞 山东省药学科学院, 山东省化学药物重点实验室, 济南
汤连升 山东省药学科学院, 山东省化学药物重点实验室, 济南
贾庆文 山东省药学科学院, 山东省化学药物重点实验室, 济南
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Abstract:
      Objective To evaluate the mutagenicity of hydrolysate of Meretrix meretrix Linnaeus soft tissue, so as to provide experimental basis for its exploitation. Methods Three mutagenicity tests were used to evaluate the mutagenic effects, including Ames test, CHL chromosome aberration assay and bone marrow micronucleus assay in mice. Results In Ames test, the revertant colonies numbers in each group were twice less than the numbers of spontaneous revertant colonies, five bacterial strains showed negative results with or without S9 activation, and the result of Ames test was negative. The CHL chromosome aberration assay and bone marrow micronucleus assay showed that the chromosome aberration rate and micronucleus rate of each dose group showed no significant difference compared with the negative control group, respectively (P>0.05). Conclusions Under this condition, the results show that all of the Ames test, chromosome aberration assay and bone marrow micronucleus assay are negative, and no mutagenicity is observed in the hydrolysate of Meretrix meretrix Linnaeus soft tissue.
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