Inhibitory effect of paeoniflorin on inflammatory chemokines and their receptor in a mouse model of asthma
Received:January 04, 2016  
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KeyWord:Bronchial asthma;Airway inflammation;Chemokines;Receptor;NF-κB;Paeoniflorin;Mouse
刘家齐 复旦大学附属华山医院, 上海
赵正晓 复旦大学附属华山医院, 上海
魏颖 复旦大学附属华山医院, 上海
徐飞 复旦大学附属华山医院, 上海
张红英 复旦大学附属华山医院, 上海
李密辉 复旦大学附属华山医院, 上海
陆林玮 复旦大学附属华山医院, 上海
魏凯 复旦大学附属华山医院, 上海
董竞成 复旦大学附属华山医院, 上海
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      Objective To study the effect of paeoniflorin on inflammatory chemokines and their receptor in a rat model of ovalbumin-induced asthma.Methods Sixty 6-week old SPF female BALB/c mice were used in this study. To establish a mouse model of asthma by sensitizing and challenging with ovalbumin.ELISA was used to analyze the serum IL-6 and TNF-α, and the specific IgE against ovalbumin (OVA-IgE), CCL19 and CCL21 in bronchoalveolar lavage fluid (BALF). RT-PCR was performed to determine CCR7mRNA and protein expression of chemokine receptor CCR7, and the level of NF-κB was tested by Western blot.ResultsIn In the paeoniflorin groups, the serum IL-6 and TNF-α levels were significantly lower, and the OVA-IgE, CCL19 and CCL21 levels in BALF were significantly reduced, compared with that in the control group.CCR7mRNA and protein expression of chemokine receptor CCR7 and NF-κB in the lung were significantly reduced by paeoniflorin.Conclusions Paeoniflorin has a remarkably inhibitory effect on the airway inflammatory chemokines CCL19/CCL21 and the receptor CCR7 in the mouse model of asthma.
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