Comparison of the differences in glutamate-induced astrocyte swelling between Wistar and Sprague-Dawley rats
Received:April 05, 2016  
View Full Text  View/Add Comment  Download reader
DOI:10.3969/j.issn.1005-4847.2016.05.003
KeyWord:Glutamate;Astrocyte;Aquaporin 4;Rats
                    
AuthorInstitution
师忠芳 首都医科大学 北京市神经外科研究所, 首都医科大学附属北京天坛医院, 北京
徐立新 首都医科大学 北京市神经外科研究所, 首都医科大学附属北京天坛医院, 北京
卢易 首都医科大学附属北京天坛医院, 北京
董丽萍 首都医科大学 北京市神经外科研究所, 首都医科大学附属北京天坛医院, 北京
闫旭 首都医科大学 北京市神经外科研究所, 首都医科大学附属北京天坛医院, 北京
杨少华 首都医科大学 北京市神经外科研究所, 首都医科大学附属北京天坛医院, 北京 ;北德克萨斯大学医学中心神经与药理系, 美国德克萨斯州沃思堡 76107
袁芳 首都医科大学 北京市神经外科研究所, 首都医科大学附属北京天坛医院, 北京
Hits: 780
Download times: 355
Abstract:
      Objective To compare the differences between the cell swelling of cultured astrocytes (AST) from Wistar and Sprague-Dawley (SD) rats after incubation with glutamate. Methods Primary cultured AST derived from the cerebral cortex of one-day-old Wistar or SD rats were prepared. The cultured AST received 1 or 10 mmol/L glutamate treatment for 48 h on the tenth day after subculture. The viability of AST was determined by lactate dehydrogenase (LDH) kit to assess the cell injury, and the perimeter of AST was measured using Image Pro Plus software after glial fibrillary acidic protein immunofluorescence staining to evaluate the astrocyte swelling. Then, the expression of aquaporin 4 (AQP4) in cultured AST was detected by quantitative reverse transcription polymerase chain reaction. Results No significant difference was found in the LDH release after the glutamate treatment in cultured AST from these two strains (P>0.05). The perimeter of AST from normal Wistar rats was shorter than that from SD rats, but was longer after the treatment of glutamate (P<0.05). Meanwhile, AQP4 expression in the Wistar rats was significantly higher than that from SD rats after incubation with 1 mmol/L glutamate (P<0.05). Conclusions These results suggeste that cultured AST from Wistar rats are more susceptible to glutamate-induced swelling than that from SD rats, and there are differences between the effects of glutamate on AQP4 expression in astrocytes of Wistar and SD rats.
Close
function PdfOpen(url){ var win="toolbar=no,location=no,directories=no,status=yes,menubar=yes,scrollbars=yes,resizable=yes"; window.open(url,"",win); } function openWin(url,w,h){ var win="toolbar=no,location=no,directories=no,status=no,menubar=no,scrollbars=yes,resizable=no,width=" + w + ",height=" + h; controlWindow=window.open(url,"",win); } &et=8C10CEA789A144B8D97FFA0DF64E418D054A31D9C62B06E56C06AE2B60CC20D372B9BE3DACCD14720CFE5277E4D027ADBF10306B07A0BB8330CF959CA56ADF9EE593F931438F96E9B61156486D041CA1AEB1FA7BE689E598C375E6F40D9201A9936ACAE77B13F606886F12E3E87ABF99&pcid=&cid=&jid=&yid=9F915C6F01DE79C5&aid=&vid=&iid=94C357A881DFC066&sid=B1989ED92BA7E896&eid=3389C664025A98F9&fileno=20160503&flag=1&is_more=0"> var my_pcid=""; var my_cid=""; var my_jid=""; var my_yid="9F915C6F01DE79C5"; var my_aid="";