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| Knockdown of tissue transglutaminase in SaOS-2 cell line inhibits its osteoblastic differentiation and mineralization |
| Received:March 08, 2016 |
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| DOI：10.3969/j.issn.1005-4847.2016.05.002 |
| KeyWord:Tissue transglutaminase;SaOS-2 cell line;Osteoblastic differerntiation |
| Author | Institution |
| 余慧镭 |
北京大学第三医院骨科, 北京 |
| 殷晓雪 |
北京大学第三医院骨科, 北京 |
| 陈仲强 |
北京大学第三医院骨科, 北京 |
| 冷慧杰 |
北京大学第三医院骨科, 北京 |
| 宋纯理 |
北京大学第三医院骨科, 北京 |
| 刘忠军 |
北京大学第三医院骨科, 北京 |
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| Abstract: |
| Objective To investigate whether TG2 plays an important role in the osteoblast differentiation and mineralization. Methods TG2 mRNA of SaOS-2 cells was knocked down using a lentivirus stably expressing short-hairpin(sh) RNA targeting TG2.Then the cells were cultured in osteo-inductive medium for 14 d to measure mineralization and for 7 d to measure the levels of osteoblastic differentiation markers including ALP activity and mRNA of collagen I, osteocalcin(OCN) and BMP-2.The wild-type SaOS-2 cells and scrambled shRNA-transducted SaOS-2 cells served as the controls. Results The controls displayed an increasing trend of the level of ALP activity and mRNA of collagen I, osteocalcin and BMP-2,and notable mineralization at 14 d.When TG2 was knocked down, ALP activity, mRNA of collagen I, osteocalcin and BMP-2 at 7d,and mineralization at 14 d were all significantly lower in comparison with the corresponding values in the controls.Conclusion TG2 is involved in the differentiation and mineralization of osteoblasts in vitro. |
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