Effect of anti-oxidant treatment on the expression and activity of SOD in diabetic rats
Received:December 08, 2015  
View Full Text  View/Add Comment  Download reader
DOI:10.3969/j.issn.1005-4847.2016.04.016
KeyWord:diabetes mellitus;N-acetylcysteine;Superoxide dismutase, SOD;Rats
              
AuthorInstitution
周玉 荆门市第一人民医院麻醉科, 湖北 荆门
王伶俐 荆门市第一人民医院麻醉科, 湖北 荆门
黄杨 荆门市第一人民医院麻醉科, 湖北 荆门
曹明香 荆门市第一人民医院麻醉科, 湖北 荆门
杨昌明 荆门市第一人民医院麻醉科, 湖北 荆门
Hits: 1200
Download times: 597
Abstract:
      Objective To study the changes of total SOD activity and expression in major organs and observe the effects of antioxidant N-acetylcysteine (NAC) on antioxidant status in diabetic rats. Methods Normal rats (group C, n=8) or streptozotocin (STZ)-induced diabetic rats (group D, n=8) were treated with NAC 1.5 g/kg/d (Group D+N, n=8) or equal volume of normal saline once daily by gastric gavage for 4 weeks. Blood samples were collected and the 15-F2t-isoprostane, total antioxidant concentration and SOD activity were assayed. The heart, lung, liver and kidney tissues were collected for assay of the total SOD activity using a kit and the protein expression of SOD isoforms, including Cu/Zn-SOD and Mn-SOD, was determined by western blot.Results The levels of 15-F2t-isoprostane and total antioxidant concentration in plasma, total SOD activity in the heart, protein expression of Cu/Zn-SOD in the heart and lung, and Mn-SOD expression in the liver were significantly increased (PPConclusions The levels of total SOD activity and protein expression of Cu/Zn-SOD and Mn-SOD in diabetic rats are tissue specific, and antioxidant NAC can attenuate the target organ damages to a certain degree by restoring the antioxidant levels in diabetic rats.
Close
function PdfOpen(url){ var win="toolbar=no,location=no,directories=no,status=yes,menubar=yes,scrollbars=yes,resizable=yes"; window.open(url,"",win); } function openWin(url,w,h){ var win="toolbar=no,location=no,directories=no,status=no,menubar=no,scrollbars=yes,resizable=no,width=" + w + ",height=" + h; controlWindow=window.open(url,"",win); } &et=FF4EC6B96077DFE724640877DE490FFEE9159DE36189A38F76CEE767BC46F00CD0908F83AD47B9417AFD821C7C0BBF88D4E97AD2FD527309457441A52983448D2D969792C2A72F0816105B6B799D893E838F629A1F3B8EF9&pcid=&cid=&jid=&yid=9F915C6F01DE79C5&aid=无法连接到远程服务器&vid=&iid=E158A972A605785F&sid=B941678158018439&eid=BA48F0B914ED890A&fileno=20160416&flag=1&is_more=0"> var my_pcid=""; var my_cid=""; var my_jid=""; var my_yid="9F915C6F01DE79C5"; var my_aid="无法连接到远程服务器";