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| Preliminary study of the inhibitory effect of selective M4 muscarinic receptor antagonist MT3 on form deprivation myopia in guinea pigs |
| Received:December 09, 2015 |
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| DOI：10.3969/j.issn.1005-4847.2016.04.013 |
| KeyWord:M4 receptor antagonist;MT3;Form deprivation myopia;TGF-β2;Gunea pigs |
| Author | Institution |
| 李涛 |
复旦大学附属金山医院, 上海 |
| 周晓东 |
复旦大学附属金山医院, 上海 |
| 李炳 |
复旦大学附属金山医院, 上海 |
| 罗秀梅 |
复旦大学附属金山医院, 上海 |
| 龚哲平 |
上海市蒙山中学, 上海 |
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| Abstract: |
| Objective To evaluate the inhibitory effect of highly selective M4 receptor antagonist MT3 on the form deprivation myopia in guinea pigs and its potential mechanism. Methods Thirty-two healthy male guinea pigs were randomly divided into three groups: control group, form deprivation group, and form deprivation+MT3 group, 8 animals in each group. Refraction was measured by retinoscopy after cycloplegia before and after the experiment. The ocular biological dimensions were measured by A-scan ultrasound. RT-PCR was used to detect the relative expression of TGF-β2 mRNA in the retina and choroid.Results Compared with the right eyes of control group, the right eyes of form deprivation+MT3 group developed relative myopia of -1.44±0.50 D (right-left eye) (P=0.001). The vitreous chamber depth and axial length of the right eyes were significantly prolonged by 0.10±0.02 mm and 0.14±0.07 mm (P<0.001, P<0.001), respectively, but the increases of myopia and axial length were significantly smaller than that of the form deprivation group (P<0.001, P<0.001, P<0.001). Down-regulation of relative mRNA expression of TGF-β2 in retina and choroid was found in the form deprivation group (P<0.001, P=0.014) compared with the right eyes of the control group, while up-regulation of relative mRNA expression of TGF-β2 in retina and choroid was found in the form deprivation+MT3 group (P<0.001, P<0.001). Conclusions MT3 can inhibit the development of form deprivation myopia in guinea pigs, which may play an important role by the regulation of TGF-β2 mRNA level in the retina and choroid. |
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