Pin1 expression in the skin and establishment of an inducible transgenic mouse model
Received:April 07, 2016  
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KeyWord:Pin1;Doxycycline;Transgenic mice;Tet-on,Skin
向建 福建医科大学癌症和神经退行性疾病转化研究省重点实验室, 福建医科大学转化医学研究院, 福州 ;福建医科大学消化道恶性肿瘤教育部重点实验室, 福州
陈鹏 福建医科大学癌症和神经退行性疾病转化研究省重点实验室, 福建医科大学转化医学研究院, 福州 ;福建医科大学消化道恶性肿瘤教育部重点实验室, 福州
张莉 福建医科大学癌症和神经退行性疾病转化研究省重点实验室, 福建医科大学转化医学研究院, 福州 ;福建医科大学消化道恶性肿瘤教育部重点实验室, 福州
卢坤平 福建医科大学癌症和神经退行性疾病转化研究省重点实验室, 福建医科大学转化医学研究院, 福州 ;福建医科大学消化道恶性肿瘤教育部重点实验室, 福州 ;哈佛医学院Beth Israel Deaconess医学中心, 医学系和肿瘤中心, 转化治疗部, 马萨诸塞州 02215 美国
廖新化 福建医科大学癌症和神经退行性疾病转化研究省重点实验室, 福建医科大学转化医学研究院, 福州 ;福建医科大学消化道恶性肿瘤教育部重点实验室, 福州
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      Objective To observe the Pin1 expression pattern in skin and to establish an inducible skin specific Pin1 overexpression mouse model.Methods The mouse Pin1 gene was cloned into modified vector pTRE2 with C-terminal Myc tag. The linearized pTRE2-Pin1 DNA was micro-injected into one-cell embryos followed by implantation into foster mice to produce TRE-Pin1 transgenic mice.Results TRE-Pin1 transgenic founder mice were successfully created. These mice were crossed with transgenic tool mice K14-rtTAto create epithelial specific double transgenic progenies. Pin1 gene was induced by incorporating doxycycline into drinking water of the mice. Pin1 protein overexpression in the skin was confirmed by Western blot and immunohistochemistry. The endogenous Pin1 protein was predominantly expressed in epidermal cells in the skin. Conclusions The inducible skin specific Pin1 overexpression mouse model is successfully established which may serve as a useful model for further study of Pin1 functions in the skin.
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