Differential expression of integrin αv,β3 in bovine endometrial epithelial cells before and after co-culture with bovine blastocysts
Received:February 29, 2016  
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DOI:10.3969/j.issn.1005-4847.2016.03.011
KeyWord:Bovine;In vivo hatched blastocyst;Co-culture;Endometrial epithelial cells;Integrin αv β3 gene
                       
AuthorInstitution
倪茜瞵 北京农学院动物科学技术学院/兽医学中医药北京市重点实验室, 北京
倪和民 北京农学院动物科学技术学院/兽医学中医药北京市重点实验室, 北京
潘兴乾 北京农学院动物科学技术学院/兽医学中医药北京市重点实验室, 北京
齐晓龙 北京农学院动物科学技术学院/兽医学中医药北京市重点实验室, 北京
盛熙晖 北京农学院动物科学技术学院/兽医学中医药北京市重点实验室, 北京
邢书涵 北京农学院动物科学技术学院/兽医学中医药北京市重点实验室, 北京
刘云海 北京农学院动物科学技术学院/兽医学中医药北京市重点实验室, 北京
郭勇 北京农学院动物科学技术学院/兽医学中医药北京市重点实验室, 北京
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Abstract:
      Objective To investigate the differential expression of integrin alpha v beta 3(αv,β3) on bovine endometrial epithelial cells before and after co-culture with bovine blastocysts, and to explore whether this specific signal might be applied as a new marker for identifying the bovine uterine receptivity. Methods The in vivo bovine embryos were co-cultured with their endometrial epithelial cells for 24 h, then, RT-PCR was used to detect the differential expression of αv,β3 among those groups. Result The results showed that αv,β3 can be expressed on bovine endometrial epithelial cells both before and after co-culture with their in vivo embryos. There were no significant differences of expression of αv,β3 between Group I (only bovine blastocyst) and the control one (P > 0.05), but there were significant differences of αv,β3 among Group Ⅱ (the hatched bovine blastocyst), and Group I, the control one (P< 0.05). Conclusions The in vivo hatched bovine blastocysts are more suitable for induction of intergrin αv,β3 in bovine endometrial epithelial cells after their co-culture than that of co-cultured with early stage blastocyst. Integrin αv,β3 might be applied as a new molecular marker for detecting bovine uterine receptive status of the bovine endometrium.
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