Objective To investigate the differential expression of integrin alpha v beta 3(αv,β3) on bovine endometrial epithelial cells before and after co-culture with bovine blastocysts, and to explore whether this specific signal might be applied as a new marker for identifying the bovine uterine receptivity. Methods The in vivo bovine embryos were co-cultured with their endometrial epithelial cells for 24 h, then, RT-PCR was used to detect the differential expression of αv,β3 among those groups. Result The results showed that αv,β3 can be expressed on bovine endometrial epithelial cells both before and after co-culture with their in vivo embryos. There were no significant differences of expression of αv,β3 between Group I (only bovine blastocyst) and the control one (P > 0.05), but there were significant differences of αv,β3 among Group Ⅱ (the hatched bovine blastocyst), and Group I, the control one (P< 0.05). Conclusions The in vivo hatched bovine blastocysts are more suitable for induction of intergrin αv,β3 in bovine endometrial epithelial cells after their co-culture than that of co-cultured with early stage blastocyst. Integrin αv,β3 might be applied as a new molecular marker for detecting bovine uterine receptive status of the bovine endometrium.