Evaluation of the detection proficiency of laboratories testing of mammalian orthoreovirus 3 antibody in laboratory mice
Received:January 13, 2016  
View Full Text  View/Add Comment  Download reader
KeyWord:Mammalian orthoreovirus 3 (Reo3);Detection capacity evaluation;ELISA;Immunofluorescence assay
王吉 中国食品药品检定研究院, 北京
付瑞 中国食品药品检定研究院, 北京
李晓波 中国食品药品检定研究院, 北京
王淑菁 中国食品药品检定研究院, 北京
卫礼 中国食品药品检定研究院, 北京
邢进 中国食品药品检定研究院, 北京
冯育芳 中国食品药品检定研究院, 北京
王洪 中国食品药品检定研究院, 北京
岳秉飞 中国食品药品检定研究院, 北京
Hits: 1371
Download times: 341
      Objective Through the proficiency validation of testing of mammalian orthoreovirus 3 (Reo3)antibody in laboratory mice, to investigate the capacity of experimental animal quality control laboratories, so as to improve the detection capacity. Methods According to the program approved by CNAS, serum samples after calibration were tested for stability and homogeneity, and numbered randomly. The random samples were issued to the participant laboratories with the Standard Operation Procedure(SOP). The participants must submit the test reports and original records in time. The feedback results were judged by the concordance rate with the anticipated results. Results 27 laboratories from 17 provinces were enrolled in this evaluation project, all of them submitted detection results on time. Results All the 27 laboratories were marked as pass or excellent, with a pass rate of 100%. ELISA was used in 26 laboratories, and immunofluorescence assay was used in one laboratory. Conclusions The ability for detection of Reo3 antibody in laboratory mice in animal test laboratories is high. The implementation of proficiency testing can reflect the inspection level of quality control laboratories.
function PdfOpen(url){ var win="toolbar=no,location=no,directories=no,status=yes,menubar=yes,scrollbars=yes,resizable=yes"; window.open(url,"",win); } function openWin(url,w,h){ var win="toolbar=no,location=no,directories=no,status=no,menubar=no,scrollbars=yes,resizable=no,width=" + w + ",height=" + h; controlWindow=window.open(url,"",win); } &et=D700F206953109B3D6C39329A22A8031AF2FB82DEDEE2DBF378B2E4222ED3C611A3963DB9ACEFC87787513D722A70D9475470EDE4E3B6E0F8CB439B474143C02350FC8EA300764614AD3483544EA2E246676BD4807899B8DE3B505608DD4739028D87506DBEF08C6E7677AE5782DF5DC2947F24AD8B28A9892B6AFE7BAEA618D&pcid=&cid=&jid=&yid=9F915C6F01DE79C5&aid=&vid=&iid=0B39A22176CE99FB&sid=DD74772618543076&eid=3E0812ED84A7B31D&fileno=20160213&flag=1&is_more=0"> var my_pcid=""; var my_cid=""; var my_jid=""; var my_yid="9F915C6F01DE79C5"; var my_aid="";