Preparation of a rat model of ulcerative colitis with spleen and kidney Yang deficiency
Received:September 17, 2015  
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DOI:10.3969/j.issn.1005-4847.2016.02.002
KeyWord:Ulcerative colitis;Spleen and kidney Yang deficiency;Enzyme linked immunosorbent assay;Rats
                       
AuthorInstitution
吴玉泓 甘肃中医药大学, 兰州 ;甘肃省中医方药挖掘与创新转化重点实验室;甘肃省中药新产品创制工程实验室, 兰州
许雅清 甘肃中医药大学, 兰州
李海龙 甘肃中医药大学, 兰州 ;甘肃省中医方药挖掘与创新转化重点实验室;甘肃省中药新产品创制工程实验室, 兰州
程小丽 甘肃中医药大学, 兰州
明海霞 甘肃中医药大学, 兰州
张磊 甘肃中医药大学, 兰州
刘峰林 甘肃中医药大学, 兰州
王瑞群 甘肃中医药大学, 兰州
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Abstract:
      Objective To establish a rat model of ulcerative colitis (UC) with spleen and kidney Yang deficiency. Methods The rat model of ulcerative colitis with spleen and kidney Yang deficiency was established by oral administration of Rhubarb decoction, intramuscular injection of hydrocortisone, and combined with ethanol enema of TNBS (2,4,6-trinitrobenzene sulfonic acid). Sixty Wistar rats (body weight 180±20 g, male:female=1:1) were randomly divided into blank control group and groups of UC models with spleen kidney Yang deficiency for 7 days, 14 days and 21days. The serum levels of FT3, FT4, and T of the rats were detected by ELISA assay. Results Compared with the blank control group, the serum levels of FT3, FT4, and T in the groups of UC rat models with spleen kidney Yang deficiency for 7 days, 14 days and 21days were decreased to a different extent (P<0.05), especially, decreased dramatically in the model group for 21 days. Conclusions FT3, FT4, and T are sensitive indexes with spleen and kidney Yang deficiency. The detection of serum levels of FT3, FT4, and T can better verify the spleen and kidney Yang deficiency in the rats, and prove that the spleen and kidney Yang deficiency type UC animal model is successfully prepared.
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