Preparation of a rat model of ulcerative colitis with spleen and kidney Yang deficiency
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    Abstract:

    Objective To establish a rat model of ulcerative colitis (UC) with spleen and kidney Yang deficiency. Methods The rat model of ulcerative colitis with spleen and kidney Yang deficiency was established by oral administration of Rhubarb decoction, intramuscular injection of hydrocortisone, and combined with ethanol enema of TNBS (2,4,6-trinitrobenzene sulfonic acid). Sixty Wistar rats (body weight 180±20 g, male:female=1:1) were randomly divided into blank control group and groups of UC models with spleen kidney Yang deficiency for 7 days, 14 days and 21days. The serum levels of FT3, FT4, and T of the rats were detected by ELISA assay. Results Compared with the blank control group, the serum levels of FT3, FT4, and T in the groups of UC rat models with spleen kidney Yang deficiency for 7 days, 14 days and 21days were decreased to a different extent (P<0.05), especially, decreased dramatically in the model group for 21 days. Conclusions FT3, FT4, and T are sensitive indexes with spleen and kidney Yang deficiency. The detection of serum levels of FT3, FT4, and T can better verify the spleen and kidney Yang deficiency in the rats, and prove that the spleen and kidney Yang deficiency type UC animal model is successfully prepared.

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History
  • Received:September 17,2015
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  • Online: April 28,2016
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