Effects of electroacupuncture on the expressions of IL-1β and ICAM-1 in bilateral brain tissues of rats with cerebral ischemia/reperfusion injury
Received:November 13, 2014  
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DOI:10.3969/j.issn.1005-4847.2015.03.011
KeyWord:Electroacupuncture;Cerebral ischemia-reperfusion injury;Interleukin-1β;Intercellular adhesion molecule-1;Rat
                       
AuthorInstitution
宋映周 北京中医药大学针灸推拿学院, 北京
孙琳琳 北京首钢医院, 北京
任暎贞 北京中医药大学针灸推拿学院, 北京
张旭辉 北京中医药大学针灸推拿学院, 北京
许明敏 北京中医药大学针灸推拿学院, 北京
于淼 北京中医药大学针灸推拿学院, 北京
郭郁 北京中医药大学针灸推拿学院, 北京
图娅 北京中医药大学针灸推拿学院, 北京
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Abstract:
      Objective To explore the variation trends of interleukin-1β (IL-1β) and intercellular adhesion molecule-1 (ICAM-1) in both normal and affected sides of brain tissues in rats with ischemia-reperfusion injury and the therapeutic action of electroacupuncture. Methods The cerebral ischemia-reperfusion model was established with suture embolization in the right middle cerebral artery. The rats were randomly divided into control group, model group and electroacupunture group. Each group was then divided into six subgroups by the time after operation (12 h,24 h,48 h,72 h,96 h,144 h), ten rats in each subgroup. Frozen sections of brain tissues were prepared and the expression of IL-1β and ICAM-1 in brain tissues of both sides were detected by immunohistochemistry. Results The expressions of IL-1β and ICAM-1 showed typical bimodal pattern in both affected ischemic region and contralateral normal region. In the model group, the peaks of IL-1β in the cerebral ischemic region were at 12 h and 48 h, while in the contralateral normal region the peaks were at 12 h and 144 h, the expression of IL-1β in the ischemic region was significantly higher than that in the contralateral normal region at 48 h (P<0.05), and lower at 96 h and 144 h (P<0.05). In the electroacupuncture group, the expressions of IL-1β in the ipsilateral region were significantly lower than that in the contralateral region at 24 h, 48 h and 144 h (P<0.05). In the model group, the peaks of ICAM-1 in the cerebral ischemic regions were at 24 h and 72 h, while in the contralateral normal regions the peaks were at 24 h and 144 h. In the electroacupuncture group, the expressions of ICAM-1 in the ischemic regions were significantly lower than that in the contralateral normal regions at all the 12 h, 24 h, 48 h, 72 h and 144 h (P<0.05). Conclusions Our findings suggest that electroacupuncture may inhibit the inflammation of ischemia/reperfusion brain tissue through reducing the expression of IL-1β and ICAM-1 to relieve the cerebral ischemia-reperfusion injury.
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