A novel rat model of atherosclerosis induced by inflammation and the therapeutic effect of Rb1
Received:June 13, 2014  
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KeyWord:Atherosclerosis;Inflammation;Zymosan;Animal model;Rb1;Rat
樊继山 重庆医科大学附属儿童医院药学部, 儿童发育疾病研究教育部重点实验室, 儿科学重庆市重点实验室, 重庆
刘丹宁 重庆医科大学附属第二医院, 重庆
何翠瑶 重庆医科大学附属儿童医院药学部, 儿童发育疾病研究教育部重点实验室, 儿科学重庆市重点实验室, 重庆
李晓辉 第三军医大学药学院, 重庆
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      Objective To establish a novel atherosclerosis model by inflammation in rats and investigate the anti-atherosclerotic effect of Rb1. Methods Healthy male SD rats were randomly divided into three groups, namely the control group, model group (using zymosan A to induce inflammation) and Rb1-treated group (12 rats in each group). The rats were administered liquid paraffin (i.p.), zymosan A (20 mg/kg, i.p., once every 4 days) or zymosan A and Rb1 (40 mg/kg, i.p., once daily), respectively. All animals were fed a high-fat diet for 10 weeks. At scheduled time points, pathological changes in the aorta were observed using Sudan IV staining and transmission electron microscopy. White blood cell count was used to assess the inflammation. The expression of NFκB, TNFα, IL6 was evaluated by real time PCR, immunohistochemistry and ELISA, respectively. Results Typical atherosclerotic changes such as fatty streaks, plaque, foam cells in the rats following zymosan A induction were alleviated by Rb1 treatment. In the Rb1-treated group, there was a markedly decreased expression of NFκB, TNFα, and IL6. Conclusion The model of atherosclerosis can be established by inflammation based on high-fat diet in rats. Rb1 inhibits atherosclerosis through anti-inflammatory effect.
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