Establishment and identification of a ClC-3/MMTV-PyMT hybrid mouse strain
Received:July 07, 2014  
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KeyWord:CLCN3;MMTV-PyMT;Transgenic mice;Breast cancer
邓璐璐 广东药学院基础学院, 广州
李琴 贵州省六盘水市人民医院, 贵州 六盘水
吴慧 广东药学院基础学院, 广州
毛建文 广东药学院基础学院, 广州
徐彬 广东药学院生命科学与生物制药学院, 广州
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      Objective To establish a CLCN3/MMTV-PyMT double transgenic mouse model of spontaneous breast tumor with simultaneously overexpressing ClC-3. Method CLCN3 transgenic mice were crossed with MMTV-PyMT spontaneous mammary tumor model mice. The genotype was determined by PCR. The expression of ClC-3 in tissues was detected by immunofluorescence and Western blot. Results CLCN3 and MMTV-PyMT transgenic mice were bred and CLCN3/MMTV-PyMT hybrid mouse model was successfully established. The ClC-3 expression in CLCN3/MMTV-PyMT hybrid mice was higher than that in the MMTV-PyMT mice, assessed by immunofluorescence and Western blot analysis. Conclusions Transgenic mouse models of spontaneous breast cancer with simultaneously overexpressing ClC-3 are successfully established. The double transgenic mice provide a good animal model for further research of ClC-3 in tumor growth and metastasis.
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