Genetic structure and diversity of T cell receptor alpha chain genes in Hezuo minipig
Received:January 16, 2014  
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KeyWord:Hezuo minipig;Gene of TCRα chain;Bioinformatics;Diversity;Genetics
高建平 中国农业科学院兰州兽医研究所, 家畜疫病病原生物学国家重点实验室, 农业部兽医公共卫生重点实验室, 兰州 ;甘肃农业大学动物医学院, 兰州
李玩生 中国农业科学院兰州兽医研究所, 家畜疫病病原生物学国家重点实验室, 农业部兽医公共卫生重点实验室, 兰州
曾爽 中国农业科学院兰州兽医研究所, 家畜疫病病原生物学国家重点实验室, 农业部兽医公共卫生重点实验室, 兰州
房永祥 中国农业科学院兰州兽医研究所, 家畜疫病病原生物学国家重点实验室, 农业部兽医公共卫生重点实验室, 兰州
冯海燕 中国农业科学院兰州兽医研究所, 家畜疫病病原生物学国家重点实验室, 农业部兽医公共卫生重点实验室, 兰州
杨孝朴 甘肃农业大学动物医学院, 兰州
景志忠 中国农业科学院兰州兽医研究所, 家畜疫病病原生物学国家重点实验室, 农业部兽医公共卫生重点实验室, 兰州 ;甘肃农业大学动物医学院, 兰州
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      Objective To analyze the complexity of molecular structure in porcine T cell receptor gene and its similarity compared to humans. Method Based on the gene of porcine T cell receptor alpha chain (TCRα) from the GenBank database, 93 swine T cell receptor alpha chain genes (STA) were cloned by reverse transcription-polymerase chain reaction (RT-PCR) from porcine peripheral blood lymphocytes, lymph nodes and spleen. Result Sequence analysis showed that STA genes all contain a domain of variable signal peptide and V, hypervariable J and conservative C. However, nucleotide sequence of STA was not completely identical with only 68.4% to 98.7% homology among genes, and had extremely sophisticated polymorphism and diversity. This was accord with the genetic structure of TCRα chain. Molecular structure, genetic evolution and classification of these genes were carried out according to the homology of TCRα gene, which all have several sites and zones of mutation on the domain of signal peptide, FR1 and CDR1, FR2 and CDR2, FR3 and CDR3. Analysis of similarity and classification of TCRα V domain(STAV)and J domain (STAJ) of Hezuo minipig using IMGT/V-QUEST tools compared with those of humans found the genetic evolution relationship that was closer, and each of TRAV and TRAJ also found to have a corresponding fragment of humans, ever in 92% of similarity of TRAV between swine and humans. Conclusion Our results indicate that inbred Hezuo minipig possesses genetic diversity against complicated environment of microbes in healthy status, and Hezuo minipig is suitable as an animal model for research on human immunology and diseases.
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