Establishment of a mouse model of orthotopic Lewis lung cancer
Received:March 28, 2014  
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KeyWord:Mouse;Lung Cancer;Animal model;Matrigel;Lewis lung cancer cells
李宁 中国医科大学附属盛京医院, 沈阳
张晓晔 中国医科大学附属盛京医院, 沈阳
蒋中秀 中国医科大学附属盛京医院, 沈阳
刘洋 中国医科大学附属盛京医院, 沈阳
李雪娇 中国医科大学附属盛京医院, 沈阳
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      Objective To establish a mouse model of orthotopic Lewis lung carcinoma using Matrigel, to evaluate the tumor growth and metastasis, and to provide a more stable mouse model of orthotopic lung cancer, which is more similar to human lung cancer. Methods Logarithmic phase of cultured Lewis lung cancer cells were suspended in Matrigel, vaccinated into the left lung of inbred C57BL/6 mice. Five mice were killed on the 4th, 7th, 10th, 13th, and 16th days, respectively, and to observe the median survival, tumor formation rate, tumor growth, and metastasis. Pathological changes of the mouse lung, liver, kidney and spleen were examined. Results In 5 mice killed on the 7th postoperative day, small tumor nodules were observed on the lung in three mice and no tumor was visible by gross inspection in the other two mice, but small tumor nodules were observed under the microscope. For all the mice killed on the 10th postoperative day, tumors were visible to the naked eye on the lung of all the five mice. On the 13th day, orthotopic tumor was observed on the lung with bloody pleural effusion and pleural metastasis in all the five mice. On the 25th day, in addition to the pleural metastasis, one mouse had pericardial metastasis and renal metastasis. The survival periods of the 5 mice were 17 d, 20 d, 22 d, 22 d, and 25 d, respectively, with a median survival period of 21.2 d (17-25 d), and the tumor formation rate was 100%. Conclusions Mouse models of orthotopic Lewis lung carcinoma is successfully established using injection of tumor cells suspended in Matrigel. This model is more similar to the growth of human lung cancer, with good stability, high tumor formation rate and characteristics of distant metastasis, therefore, is worthy of further application.
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