Differential expression of CFTR gene in the mouse intestinal tissues
Received:April 09, 2014  
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DOI:10.3969/j.issn.1005-4847.2014.05.011
KeyWord:CFTR;Mouse;Intestine;CFTR gene expression;Fluorescence quantitative PCR
              
AuthorInstitution
王月影 河南农业大学农业部动物生化与营养重点开放实验室, 郑州
韩莹倩 河南农业大学农业部动物生化与营养重点开放实验室, 郑州
查光明 河南农业大学农业部动物生化与营养重点开放实验室, 郑州
汪新建 河南农业大学农业部动物生化与营养重点开放实验室, 郑州
李和平 河南农业大学农业部动物生化与营养重点开放实验室, 郑州
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Abstract:
      Object This experiment was conducted to study the relationship between CFTR gene expression in the intestinal tissues and secretory diarrhea. Methods Twenty-four Kunming mice were selected, half male and half female, and were randomly divided into 3 groups (n=8 in each group): control group with intraperitoneal injection of 0.2 mL normal saline, and the experimental group of mice by intraperitoneal injection of lipopolysaccharide(LPS) (6 mg/kg·bw). The mental state and intestinal morphology of the mice at 1 h and 8 h after LPS injection were observed to assess whether the secretory diarrhea model was successfully established. The expression of CFTR gene segments of intestine tissue was detected by fluorescence quantitative PCR. Results LPS induced secretory diarrhea. CFTR gene was expressed in the mouse duodenum, jejunum, ileum and colon tissues with different expression abundance. It was highest in the colon, but the difference was not significant between intestinal segments. Compared with the control group, LPS up-regulated the transcription level of CFTR gene in the duodenum, jejunum and ileum, and down-regulated the transcription of CFTR gene in the colon. Conclusions The results of our study suggest that the changes of the transcriptional level of CFTR gene are closely related with the diarrhea induced by LPS and the effects in different intestinal segments on the diarrhea is different. The jejunum plays a crucial role and the colon plays a least role in the Cl- secretion.
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