Establishment of an anti-CD45 monoclonal antibody that inhibits T-cell proliferation
Received:January 10, 2014  
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DOI:10.3969/j.issn.1005-4847.2014.05.008
KeyWord:CD45;Monoclonal antibody;Inhibition;T-cell proliferation;Mouse;Rat;Dendritic cells
                    
AuthorInstitution
苏泽红 南华大学药学与生物科学学院, 湖南 衡阳
李亚林 南华大学实验动物学部, 湖南 衡阳
谭翔文 南华大学实验动物学部, 湖南 衡阳
余坚 南华大学实验动物学部, 湖南 衡阳
刘鑫 南华大学实验动物学部, 湖南 衡阳
袁月亲 南华大学药学与生物科学学院, 湖南 衡阳
练高建 南华大学实验动物学部, 湖南 衡阳
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Abstract:
      Objective To identify molecules that modulate T-cell functions and serve the studies on T-cell mediated autoimmune diseases. Methods Bone marrow-derived dentritic cells were collected from BALB/c mice to immunize Wistar rats, and to establish many hybridoma cell lines. Many hybridoma cell lines which could modulate T-cell functions were obtained. One of the cell lines, most actively inhibiting T-cell proliferation, was further studied. Results The anti-CD45 mAb recognized CD45 and significantly suppressed T-cell proliferation in proliferation assays. Conclusions Our results indicate that the anti-CD45 mAb can effectively suppress T-cell proliferation, and is promising to be used in the prevention and treatment of T-cell mediated autoimmune diseases in the future.
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