Establishment of an anti-CD45 monoclonal antibody that inhibits T-cell proliferation
Received:January 10, 2014  
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KeyWord:CD45;Monoclonal antibody;Inhibition;T-cell proliferation;Mouse;Rat;Dendritic cells
苏泽红 南华大学药学与生物科学学院, 湖南 衡阳
李亚林 南华大学实验动物学部, 湖南 衡阳
谭翔文 南华大学实验动物学部, 湖南 衡阳
余坚 南华大学实验动物学部, 湖南 衡阳
刘鑫 南华大学实验动物学部, 湖南 衡阳
袁月亲 南华大学药学与生物科学学院, 湖南 衡阳
练高建 南华大学实验动物学部, 湖南 衡阳
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      Objective To identify molecules that modulate T-cell functions and serve the studies on T-cell mediated autoimmune diseases. Methods Bone marrow-derived dentritic cells were collected from BALB/c mice to immunize Wistar rats, and to establish many hybridoma cell lines. Many hybridoma cell lines which could modulate T-cell functions were obtained. One of the cell lines, most actively inhibiting T-cell proliferation, was further studied. Results The anti-CD45 mAb recognized CD45 and significantly suppressed T-cell proliferation in proliferation assays. Conclusions Our results indicate that the anti-CD45 mAb can effectively suppress T-cell proliferation, and is promising to be used in the prevention and treatment of T-cell mediated autoimmune diseases in the future.
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