Cloning and analysis of tissue expression of PGC-1α gene in Guangxi Bama mini-pigs
Received:April 25, 2014  
View Full Text  View/Add Comment  Download reader
DOI:10.3969/j.issn.1005-4847.2014.05.006
KeyWord:Guangxi Bama mini-pig;PGC-1α gene;Sequencing analysis;Tissue expression
                 
AuthorInstitution
严雪瑜 广西大学动物科学技术学院, 南宁
敖秋桅 广西大学动物科学技术学院, 南宁
严小东 广西防城港市畜牧站, 广西 防城港
蒋钦杨 广西大学动物科学技术学院, 南宁
郭亚芬 广西大学动物科学技术学院, 南宁
兰干球 广西大学动物科学技术学院, 南宁
Hits: 978
Download times: 570
Abstract:
      Objective To clone the coding sequence of Guangxi Bama mini-pig PGC-1α gene, and to analyze the expression of PGC-1α gene in various tissues of mini-pigs using RT-PCR and QRT-PCR techniques. Methods The PGC-1α gene coding sequence (CDS) was amplified by PCR from the cDNA of longissimus muscle of Guangxi Bama mini-pig. The PCR products were inserted into pEASY-T5 vector, transfected E. coli, identified and sequenced. The PGC-1α gene expression in different tissues of the Bama mini-pigs was detected by RT-PCR and QRT-PCR assays. Results The PGC-1α gene CDS of Guangxi Bama mini-pig was cloned. It was 2391 bp in length. It had 99.9% homology with the reference sequence, and had two synonymous mutations that were C-A1105 and G-A1524. The expression level of PGC-1α gene was higher in the heart and kidney, followed by liver, subcutaneous fat and longissimus muscle, but the expression was not detected in pancreas of Guangxi Bama mini-pig. Conclusions We have successfully cloned the PGC-1α gene of Guangxi Bama mini-pig, and detected this gene expression in six tissues. The results of this study will provide a basis for studying the effect of PGC-1α on type 2 diabetes mellitus (T2DM) in Bama mini-pigs.
Close
function PdfOpen(url){ var win="toolbar=no,location=no,directories=no,status=yes,menubar=yes,scrollbars=yes,resizable=yes"; window.open(url,"",win); } function openWin(url,w,h){ var win="toolbar=no,location=no,directories=no,status=no,menubar=no,scrollbars=yes,resizable=no,width=" + w + ",height=" + h; controlWindow=window.open(url,"",win); } &et=4317B089317DAFE2F37D860DFF8DE2BEE2786B306329C2179DB7DBD6D0F77CA1B400724EB38D781DCEE942264C10ACA9956C4E81EA4FFF5F90AA0850E97EBF8CB20BABB1F4AFCE678FC271E6AE20438FACD5ABA3F3AD9570&pcid=&cid=&jid=&yid=9EAD63ADE6B277ED&aid=&vid=&iid=94C357A881DFC066&sid=DB817633AA4F79B9&eid=4AD960B5AD2D111A&fileno=20140506&flag=1&is_more=0"> var my_pcid=""; var my_cid=""; var my_jid=""; var my_yid="9EAD63ADE6B277ED"; var my_aid="";