Cloning and analysis of tissue expression of PGC-1α gene in Guangxi Bama mini-pigs
Received:April 25, 2014  
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KeyWord:Guangxi Bama mini-pig;PGC-1α gene;Sequencing analysis;Tissue expression
严雪瑜 广西大学动物科学技术学院, 南宁
敖秋桅 广西大学动物科学技术学院, 南宁
严小东 广西防城港市畜牧站, 广西 防城港
蒋钦杨 广西大学动物科学技术学院, 南宁
郭亚芬 广西大学动物科学技术学院, 南宁
兰干球 广西大学动物科学技术学院, 南宁
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      Objective To clone the coding sequence of Guangxi Bama mini-pig PGC-1α gene, and to analyze the expression of PGC-1α gene in various tissues of mini-pigs using RT-PCR and QRT-PCR techniques. Methods The PGC-1α gene coding sequence (CDS) was amplified by PCR from the cDNA of longissimus muscle of Guangxi Bama mini-pig. The PCR products were inserted into pEASY-T5 vector, transfected E. coli, identified and sequenced. The PGC-1α gene expression in different tissues of the Bama mini-pigs was detected by RT-PCR and QRT-PCR assays. Results The PGC-1α gene CDS of Guangxi Bama mini-pig was cloned. It was 2391 bp in length. It had 99.9% homology with the reference sequence, and had two synonymous mutations that were C-A1105 and G-A1524. The expression level of PGC-1α gene was higher in the heart and kidney, followed by liver, subcutaneous fat and longissimus muscle, but the expression was not detected in pancreas of Guangxi Bama mini-pig. Conclusions We have successfully cloned the PGC-1α gene of Guangxi Bama mini-pig, and detected this gene expression in six tissues. The results of this study will provide a basis for studying the effect of PGC-1α on type 2 diabetes mellitus (T2DM) in Bama mini-pigs.
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