Effect of caspase-9 inhibitor on apoptosis in cartilage endplate chondrocytes in rat vertebrae
Received:June 03, 2014  
View Full Text  View/Add Comment  Download reader
KeyWord:Caspase-9 inhibitor;Fetal bovine serum;Cartilage endplate;Apoptosis Chondrocytes;Vertebra;Rat
卢伟 复旦大学附属金山医院骨科, 上海
李德芳 复旦大学附属金山医院骨科, 上海
朱斌 安徽医科大学第二附属医院骨科, 合肥
吴靖平 复旦大学附属金山医院骨科, 上海
Hits: 761
Download times: 507
      Objective To explore the effect of caspase-9 inhibitor on low fetal bovine serum (FBS)-induced apoptosis in cartilage endplate chondrocytes in SD rat vertebrae. Methods Disc cartilage endplates were obtained from 3-month old SD rats and subjected to sequential digestion to harvest chondrocytes for primary culture, and apoptosis was induced by 1% FBS for 48 hours. Three groups of chondrocytes were treated by 1% FBS, caspase-9 inhibitor (Z-LEHD-FMK) and DMSO, respectively. After 48 hours, apoptosis was detected by DAPI staining and flow cytometry. The expression of procaspase-9, active caspase-9 and active caspase-3 was monitored by Western blot. Results Compared with the 1% FBS group (40.8±0.84)% and DMSO group (40.2±1.56)%, the apoptosis rate of the caspase-9 inhibitor group (26.3±2.56)% was significantly lower (P<0.05). The expressions of active caspase-9 and active caspase-3 in the caspase-9 inhibitor group were significantly lower than those in the other two groups (P<0.05). Conclusions Caspase-9 inhibitor can inhibit low FBS-induced apoptosis in cartilage endplate chondrocytes of rat vertebrae, and might become a new drug for the treatment of disc degeneration.
function PdfOpen(url){ var win="toolbar=no,location=no,directories=no,status=yes,menubar=yes,scrollbars=yes,resizable=yes"; window.open(url,"",win); } function openWin(url,w,h){ var win="toolbar=no,location=no,directories=no,status=no,menubar=no,scrollbars=yes,resizable=no,width=" + w + ",height=" + h; controlWindow=window.open(url,"",win); } &et=FF4EC6B96077DFE781F4A61483CA646190C88CB09020250EDDDFCFC937DFA1169C1DF4DEC8EAF58BF7E20846D2C5F228A39CB1B38E65B8F2E908A5D824CB3C3471042800762C87A45108798B852DBD675EA63E7D09FD7275C69B3681D0C2A819&pcid=&cid=&jid=&yid=9EAD63ADE6B277ED&aid=&vid=&iid=94C357A881DFC066&sid=BCA2697F357F2001&eid=659D3B06EBF534A7&fileno=20140504&flag=1&is_more=0"> var my_pcid=""; var my_cid=""; var my_jid=""; var my_yid="9EAD63ADE6B277ED"; var my_aid="";