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| Direct activation of expression vector with β-casein gene promoter using engineered TALE transcription factors in mouse fibroblasts |
| Received:May 03, 2014 |
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| DOI：10.3969/j.issn.1005-4847.2014.05.003 |
| KeyWord:Transcription activator-like effector-transcription factors (TALE-TFs);Mouse fibroblasts;β-casein gene promoter;Gene expression modulation |
| Author | Institution |
| 皮文辉 |
新疆生产建设兵团绵羊繁育生物技术重点实验室, 新疆 石河子 |
| 梁龙 |
新疆埃乐欣药业有限公司, 乌鲁木齐 |
| 唐红 |
新疆生产建设兵团绵羊繁育生物技术重点实验室, 新疆 石河子 |
| 张译元 |
新疆生产建设兵团绵羊繁育生物技术重点实验室, 新疆 石河子 |
| 郭延华 |
新疆生产建设兵团绵羊繁育生物技术重点实验室, 新疆 石河子 |
| 王立民 |
新疆生产建设兵团绵羊繁育生物技术重点实验室, 新疆 石河子 |
| 向春和 |
石河子大学动物科技学院, 新疆 石河子 |
| 周平 |
新疆生产建设兵团绵羊繁育生物技术重点实验室, 新疆 石河子 |
| 刘守仁 |
新疆生产建设兵团绵羊繁育生物技术重点实验室, 新疆 石河子 |
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| Abstract: |
| Objective TALE-TFs were adopted to provide a new way in detection of the expression result of β-casein gene promoter-interesting gene expression cassettes in mouse fibroblasts. Methods TALE-TFs of eukaryotic expression plasmid and expression cassette with β-casein gene promoter and red fluorescent protein reporter gene were co-nucleofected into mouse fibroblasts by Amaxa nucleofector. Results and Conclusion β-casein gene promoter was activated by artificial TALE-TFs in the mouse fibroblasts. The way is a new expression verification system instead of mammary epithelial cells with fibroblasts. |
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