Direct activation of expression vector with β-casein gene promoter using engineered TALE transcription factors in mouse fibroblasts
Received:May 03, 2014  
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DOI:10.3969/j.issn.1005-4847.2014.05.003
KeyWord:Transcription activator-like effector-transcription factors (TALE-TFs);Mouse fibroblasts;β-casein gene promoter;Gene expression modulation
                          
AuthorInstitution
皮文辉 新疆生产建设兵团绵羊繁育生物技术重点实验室, 新疆 石河子
梁龙 新疆埃乐欣药业有限公司, 乌鲁木齐
唐红 新疆生产建设兵团绵羊繁育生物技术重点实验室, 新疆 石河子
张译元 新疆生产建设兵团绵羊繁育生物技术重点实验室, 新疆 石河子
郭延华 新疆生产建设兵团绵羊繁育生物技术重点实验室, 新疆 石河子
王立民 新疆生产建设兵团绵羊繁育生物技术重点实验室, 新疆 石河子
向春和 石河子大学动物科技学院, 新疆 石河子
周平 新疆生产建设兵团绵羊繁育生物技术重点实验室, 新疆 石河子
刘守仁 新疆生产建设兵团绵羊繁育生物技术重点实验室, 新疆 石河子
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Abstract:
      Objective TALE-TFs were adopted to provide a new way in detection of the expression result of β-casein gene promoter-interesting gene expression cassettes in mouse fibroblasts. Methods TALE-TFs of eukaryotic expression plasmid and expression cassette with β-casein gene promoter and red fluorescent protein reporter gene were co-nucleofected into mouse fibroblasts by Amaxa nucleofector. Results and Conclusion β-casein gene promoter was activated by artificial TALE-TFs in the mouse fibroblasts. The way is a new expression verification system instead of mammary epithelial cells with fibroblasts.
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