Effect of CD82 on the expression of integrin αV, β3, E-cadherin and β-catenin in uterine epithelial cells in pregnant mice
Received:January 20, 2014  
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DOI:10.3969/j.issn.1005-4847.2014.03.012
KeyWord:CD82 gene;Recombinant adenovirus;Uterine epithelial cells;Primary cell culture;Pregnant mice
              
AuthorInstitution
尉晓蔚 大连大学附属中山医院, 大连
谭冬梅 重庆医科大学实验动物中心, 重庆
张倩 重庆医科大学实验动物中心, 重庆
何通川 美国芝加哥大学分子肿瘤实验室
谭毅 重庆医科大学实验动物中心, 重庆
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Abstract:
      Objective Uterine epithelial cells were isolated from pregnant mice and cultured in vitro, and examined the effect of CD82 on the expression of integrin αV, β3, E-cadherin and β-catenin in the cells. Methods The uterine epithelial cells were primarily isolated from pregnant mouse uterus. The recombinant adenovirus containing mouse CD82 gene which had been constructed in our lab infected the uterine epithelial cells. Immunocytochemistry was used to detect the protein expressions of integrin αV, β3, E-cadherin and β-catenin in the uterine epithelial cells, which were infected with CD82 adenovirus or not. Results 1. The purity of primary cultured cells was (93.2 ± 0.6)%. 2. The transfection efficiency of CD82 recombinant adenovirus was (92±4.5)%. The adenoviral particles carrying CD82 gene indeed expressed CD82 gene and protein in the primary uterine epithelial cells after 24 hours or 48 hours. 3. The uterine epithelial cells of pregnant mice on d4 expressed integrin αV, β3, E-cadherin and β-catenin. 4. In contrast to the control group, when CD82 adenovirus infected cells, the uterine epithelial cells of pregnant mice on d4 increased the expression of integrin αV, β3 and β-catenin protein, had no significant changes of E-cadherin. Conclusions CD82 may have effect on the expression of integrin αV, β3 and β-catenin in mouse uterine epithelial cells before implantation.
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