Comparison of the effect of three cryoprotectants on vitrification-cryopreservation of mouse epididymis
Received:September 19, 2013  
View Full Text  View/Add Comment  Download reader
DOI:10.3969/j.issn.1005-4847.2014.02.014
KeyWord:Mouse;Epididymis;Cryoprotectant solution;Freezing efficiency;Vitrification
                 
AuthorInstitution
李淼 中国科学院上海生命科学研究院, 上海
郁丽丽 上海斯莱克实验动物有限责任公司, 上海
张艺宝 南京农业大学动物医学系, 南京
强苏静 上海斯莱克实验动物有限责任公司, 上海
刘丽均 上海斯莱克实验动物有限责任公司, 上海
徐平 中国科学院上海生命科学研究院, 上海 ;上海斯莱克实验动物有限责任公司, 上海
Hits: 991
Download times: 546
Abstract:
      Objective To compare the effect of three cryoprotectants on vitrification-cryopreservation of C57BL/6J mouse epididymis. Method Epididymises from 6-8-week old male C57BL/6J mice (age) were cryopreserved using DMSO, PROH and R18S3 cryoprotectant solution and thawed, respectively. The morphology of sperm from thawed epididymis, the rate of post-thawing survival and reproductive capacity were determined to evaluate the freezing efficiency of the three cryoprotectants. Results The sperms from thawed epididymis of the three groups were all well-preserved structurally. The survival rate of sperms was 88.17%, 61.17% and 16.83% in the PROH, DMSO and R18S3 cryoprotectant solutions, respectively, and there were significant differences between the three cryopreservation groups (P<0.05 for all). The number of pups from the DMSO, PROH and R18S3 groups were 13, 8 and 17 mice after ICSI and embryo implantation, respectively. Conclusions All the three cryoprotectants DMSO, PROH and R18S3 solutions are suitable for vitrification-cryopreservation of C57BL/6J mouse epididymis. But PROH is the preference of these cryoprotectant solutions.
Close
function PdfOpen(url){ var win="toolbar=no,location=no,directories=no,status=yes,menubar=yes,scrollbars=yes,resizable=yes"; window.open(url,"",win); } function openWin(url,w,h){ var win="toolbar=no,location=no,directories=no,status=no,menubar=no,scrollbars=yes,resizable=no,width=" + w + ",height=" + h; controlWindow=window.open(url,"",win); } &et=8C10CEA789A144B8D97FFA0DF64E418D5E72FC91AAF1ABC6AFFA0011489CEDFCCFDCB916FCD3C8A4AB3BD6D4457850820C7AC86D43CB8468BA193F890085F206279A938CF0D75197786741D5352B9E32F28496812487558D3D33620FF493D72D9FAC7F9A00794830&pcid=&cid=&jid=&yid=9EAD63ADE6B277ED&aid=&vid=&iid=0B39A22176CE99FB&sid=95D537AC89B28832&eid=8BD23BD67BF01A5C&fileno=20140215&flag=1&is_more=0"> var my_pcid=""; var my_cid=""; var my_jid=""; var my_yid="9EAD63ADE6B277ED"; var my_aid="";