|
| Comparison of the rat models of liver cancer spleen deficiency established by Dachengqi and Xiaochengqi decoctions |
| Received:October 28, 2013 |
| View Full Text View/Add Comment Download reader |
| DOI：10.3969/j.issn.1005-4847.2014.02.005 |
| KeyWord:Spleen deficiency;Liver cancer;Dachengqi decoction;Xiaochengqi decoction;Animal model;Rat |
| Author | Institution |
| 孙保国 |
中山大学附属第一医院, 广州 |
| 项婷 |
中山大学附属第一医院, 广州 |
| 李玉龙 |
中山大学附属第一医院, 广州 |
| 陈泽雄 |
中山大学附属第一医院, 广州 |
|
| Hits: 4579 |
| Download times: 1844 |
| Abstract: |
| Objective To explore the establishing methods and differences of rat models of spleen deficiency and spleen deficiency liver cancer using the traditional Chinese medicine Dachengqi and Xiaochengqi decoctions.Methods Spleen-deficiency rat models were developed by multifactor methods: bitter-cold purgation (Dachengqi or Xiaochengqi decoction), cold-wet environment, tiredness, and fasting on alternate days for 30 days. Seven days after spleen-deficiency modeled,liver cancer in the spleen-deficiency rats and normal rats was developed by subcutaneously inoculation of Walker-256 carcinoma cell line in nude mice and then transplanted into rat livers. Liver cancer models were observed for 35 days. Sixty 3-week old male Wistar rats were randomly distributed into 4 groups: normal group, liver cancer model group, and Dachengqi and Xiaochengqi decoction groups. Degree of spleen deficiency, changes of the body-weight, survival time and tumor formation were recorded. Results Spleen deficiency rat models were successfully established. The weight gain of rats in the spleen-deficiency groups was significantly inhibited (P<0.01), and during the first 20 days (but not later) the average body weight of the Dachengqi decoction group was significantly higher than that of the Xiaochengqi decoction group (P<0.05). Spleen-deficiency scores of rats in the Xiaochengqi and Dachengqi groups were higher than those in the blank tumor group, especially in the Xiaochengqi group (P<0.01). The total tumor formation rate was 91.1% and 80% in the blank tumor groups, and 93.3% in both Xiaochengqi and Dachengqi groups, respectively. The average survival time of Xiaochengqi group was lower than that of the blank tumor and Dachengqi groups (P<0.01 and P<0.05). The cumulative survival rate of the Xiaochengqi group and rats with a higher spleen-deficiency score was lower than that of the other groups (P<0.05). Conclusions Xiaochengqi decoction may induce spleen deficiency more seriously than Dachengqi decoction, and spleen deficiency may be an important unfavorable prognostic factor for rat models of liver cancer. |
| Close |
|
|
|