Pathological changes of the kidneys in mouse models of adriamycin-induced-nephrosis
Received:October 14, 2013  
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KeyWord:Adriamycin nephropathy;Mice;Kidney
刘练 重庆医科大学附属儿童医院肾脏免疫科、儿童发育疾病研究教育部重点实验室、儿科学重庆市重点实验室、重庆市儿童发育重大疾病诊治与预防国际科技合作基地, 重庆
张高福 重庆医科大学附属儿童医院肾脏免疫科、儿童发育疾病研究教育部重点实验室、儿科学重庆市重点实验室、重庆市儿童发育重大疾病诊治与预防国际科技合作基地, 重庆
李秋 重庆医科大学附属儿童医院肾脏免疫科、儿童发育疾病研究教育部重点实验室、儿科学重庆市重点实验室、重庆市儿童发育重大疾病诊治与预防国际科技合作基地, 重庆
王墨 重庆医科大学附属儿童医院肾脏免疫科、儿童发育疾病研究教育部重点实验室、儿科学重庆市重点实验室、重庆市儿童发育重大疾病诊治与预防国际科技合作基地, 重庆
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      Objective Our purpose was to observe the renal pathological changes in the mouse modells of adriamycin-induced nephropathy in different periods. Method 48 healthy male BALB/c mice were randomly divided into control group and model group.The model group received a disposable tail vein injection of adriamycin 10.5 mg/kg body weight, and the control group received the same amount of saline. 24-hour urinary protein, serum biochemical indexes and kidney pathological changes were dynamically observed for 12 weeks. Results Proteinuria of model mice appeared in the 2th week after ADR injection, which lasted to the end of the 12-week experiment, At the 8th week, the amount of urine protein reached a peak (P<0.05); The serum albumin was decreased at the 4th week, cholesterol was increased at 8th week. At the end of experiment, serum creatinine was also increased (P<0.05). Minimal change nephrotic syndrome (MCNS) was observed in model mice at the 4th week; the lesions in renal tissues at 8th weeks were more serious than that at 4th weeks, but glomerular sclerosis was unconspicuous.Focal segmental glomerulonephritis (FSGS) was seen at the 12th week. The GSI of the model mice was(2.81±0.84)%, significantly higher than that of the control mice ((0.33±0.21)% ) at 12th week(P<0.01). Conclusions A mouse model with adriamycin-induced-nephrosis can be successfully established by a disposable tail vein injection of adriamycin in a dose of 10.5 mg/kg body weight. The early manifest ation of this model is MCNS, and at a late stage, it may be changed into FSGS.
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